Op de reukbol gericht QD-bioconjugaat en Kv1.3-blokkerende peptide verbeteren de metabole gezondheid bij zwaarlijvige mannelijke muizen

Het reuksysteem is een aanjager van het voedingsgedrag, waarbij de reukscherpte wordt gemoduleerd door de metabolische toestand van het individu. De prikkelbaarheid van de belangrijkste outputneuronen van de bulbus olfactorius (OB) kan worden gemoduleerd door zich te richten op een spanningsafhankelijk kaliumkanaal, Kv1.3, dat reageert op veranderingen in metabole factoren zoals insuline, glucose en glucagon-achtig peptide-1 .

Omdat is vastgesteld dat gengerichte deletie of remming van Kv1.3 in de periferie het energiemetabolisme verhoogt en het lichaamsgewicht verlaagt, veronderstelden we dat remming van Kv1.3 selectief in de OB de prikkelbaarheid van de outputneuronen zou kunnen verbeteren om veranderingen in energie op te roepen. homeostase. We hebben daarbij metaal-histidine-coördinatie gebruikt om de Kv1.3-remmer margatoxine (MgTx) zelf te assembleren tot fluorescerende kwantumdots (QDMgTx) als een middel om cellen in vivo te labelen en veranderingen in neuronale prikkelbaarheid en metabolisme te testen wanneer ze aan de OB worden afgeleverd.

Gebruik patch-clamp elektrofysiologie te Kv1.3 eigenschappen meten heteroloog tot expressie cellen en natieve mitrale cellen OB plakken, vonden we dat QDMgTx hadden een hoge snelheid van remming, maar met een lagere IC _50,  en verhoogde actiepotentiaal spuitfrequentie. QDMgTx was in staat om gekloonde Kv1.3-kanalen te labelen, maar was niet zichtbaar bij levering aan native Kv1.3 in de OB. Door voeding geïnduceerde zwaarlijvige muizen bleken het lichaamsgewicht te verminderen en glucose sneller te verwijderen na osmotische minipompafgifte van QDMgTx/MgTx aan de verloskundige, en na toediening van MgTx verhoogden ze het gebruik van vetten als brandstof (verminderde respiratoire uitwisselingsratio). Deze resultaten suggereren dat een verbeterde prikkelbaarheid van bulbaire output-neuronen metabolische reacties kan aansturen.

Verbeterde veiligheid en anti-tumor werkzaamheid van schakelbare dubbele chimere antigeenreceptor-engineered T-cel tegen solide tumor door middel van een synthetisch bifunctioneel PD-L1-blokkerend peptide

Door chimere antigeenreceptor (CAR) ontwikkelde T-cellen (CAR-Ts)-therapie heeft een uitstekende werkzaamheid bij de behandeling van kanker, met name de indrukwekkende resultaten bij hematologische maligniteiten. Helaas wordt de toepassing ervan op solide tumoren uitgedaagd door de off-target effecten veroorzaakt door het ontbreken van tumorspecifieke antigenen en de immunosuppressie veroorzaakt door de micro-omgeving van de tumor.

We construeerden een schakelbare dubbele receptor CAR-T-cel (sdCAR-T) waarvan de activiteit was gebaseerd op dubbele antigenen (mesotheline en fluoresceïne-isothiocyanaat) en strikt werd gecontroleerd door een “switch” (FPBM) bestaande uit een PD-L1-blokkerend peptide geconjugeerd aan fluoresceïne isothiocyanaat. SdCAR-T-cellen werden alleen geactiveerd wanneer FPBM en verwante tumorcellen die zowel PD-L1 als mesotheline tot expressie brengen, naast elkaar bestaan. Belangrijk is dat langdurige proliferatie-experimenten in vitro en farmacodynamisch onderzoek in vivo een sterkere antitumoractiviteit van dit systeem aantoonden in vergelijking met de tweede generatie mesotheline CAR-T-cellen. Aangezien dit nieuwe behandelingsparadigma veiliger en effectiever was dan traditionele CAR-T-cellen, kan het een nieuwe strategie worden voor de behandeling van solide tumoren.

 

Gerichte blokkering van TGF-β-receptor I-bindingsplaats met behulp van op maat gemaakte peptidesegmenten om de signaalroute te remmen

Achtergrond:  TGF-β-isovormen spelen een cruciale rol in diverse cellulaire processen. Daarom biedt het targeten en remmen van de TGF-β-signaleringsroute een potentiële therapeutische mogelijkheid. TGF-β-isovormen binden en brengen de receptoren (TβRII en TβRI) op een geordende manier samen om een ​​signaalcomplex te vormen.

Doelstellingen:  Hierin is een antagonistische variant van TGF-β (AnTβ) ontworpen en bereid om de vorming van een signaalcomplex en bijgevolg de signaalroute ervan te remmen. Deze TGF-β homodimere variant bevat intacte TβRII-bindingsplaatsen en geblokkeerde TβRI-bindingsplaatsen door drie peptidesegmenten te vervangen. Dus AnTβ kon alleen binden aan TβRII, maar binding en rekrutering van TβRI voorkomen om een ​​signaleringscomplex te vormen.

Materialen en methoden:  Een betrouwbaar model van AnTβ werd gebouwd en verfijnd met behulp van moleculaire dynamica (MD) simulatie, gevolgd door het onderzoeken van de interacties van AnTβ met de receptoren met behulp van in silico docking-onderzoeken. Na expressie van disulfide-gebonden AnTβ in een SHuffle-stam en zuivering van het eiwit met behulp van affiniteitschromatografie, werd de biologische activiteit ervan geëvalueerd met behulp van Mink-longepitheelcellen (Mvl Lu).

Resultaten:  Er werden geen significante significante veranderingen in de AnTβ-structuur waargenomen in vergelijking met het natieve eiwit. Op basis van de koppelingsanalyse bindt AnTβ aan TβRII vergelijkbaar met TGF-β en de binding aan TRI was aanzienlijk verminderd, wat consistent was met ons ontwerpdoel. Op cellen gebaseerde bioassay gaf aan dat AnTβ door TGF-β geïnduceerde remming van celgroei zou kunnen moduleren.

Conclusies:  Onze analyse suggereert dat de antagonistische potentie van AnTβ kan worden gebruikt als een anti-TGFβ-signaleringsfactor in de toekomstperspectieven.

Van erwt afgeleide peptiden, VLP, LLP, VA en LL, verbeteren de insulineresistentie in HepG2-cellen door IRS-1/PI3K/AKT te activeren en ROS-gemedieerde p38MAPK-signalering te blokkeren

Deze studie evalueerde het effect van vier peptiden, VLP, LLP, LL en LL van erwt op het reguleren van glucosemetabolisme en antioxidant via IRS-1/PI3K/AKT en p38MAPK-signaalroute in IR-HepG2- cellen geïnduceerd door ^10-6  M insuline. De genenexpressie van PEPCK, G6Pase, GLUT2 en IRS-1 en eiwitten van IRS-1, p(Ser307)-IRS-1, AKT, p(Ser473)-AKT, p38MAPK en p-p38MAPK werden bepaald door RT- PCR en western blotting, respectievelijk.

De resultaten laten zien dat ze zeer krachtig zijn op het stimuleren van het glucosemetabolisme en het verlichten van oxidatieve stress in IR-HepG2-cellen. VLP, LLP, VA en LL verminderden Ser307- fosforylering van IRS-1 en bevorderden Ser473-fosforylering van AKT. Onder hen verhoogden LLP, VA en LL de expressie van zowel het gen als het eiwit van GLUT2, en VLP en LL verminderden p38MAPK-fosforylering en vertoonden een sterke antioxidantcapaciteit.

Daarom hebben erwten-oligopeptiden een aanzienlijk potentieel voor het omkeren van de metabole afwijkingen die gepaard gaan met type 2-diabetes. PRAKTISCHE TOEPASSINGEN: Dit artikel onderzocht het interventie-effect van VLP, LLP, VA en LL dat van erwt op insulineresistentie, en de mechanismen werden gedetecteerd door western blotting. De resultaten bieden theoretische kennis voor de preventie van insulineresistentie in T2D van erwt-afgeleide peptiden en leggen de basis voor de ontwikkeling van functionele producten en medicijnen in de toekomst.

Blokkering van de PD-1/PD-L1-interactie door een nieuwe cyclische peptideremmer voor kankerimmunotherapie

Door de interactie van PD-1/PD-L1 kunnen tumorcellen ontsnappen aan immuunsurveillance. Klinisch succes van de antilichaamgeneesmiddelen heeft bewezen dat blokkade van de PD-1/PD-L1-route een veelbelovende strategie is voor immunotherapie tegen kanker. Hier hebben we een cyclisch peptide C8 ontwikkeld met behulp van Ph.D.-C7C-faagweergavetechnologie.

C8 vertoonde een hoge bindingsaffiniteit met hPD-1 en zou de interactie van PD-1/PD-L1 effectief kunnen verstoren. Verder zou C8  de activering van CD8 ⁺ -T-cellen in mononucleaire cellen van menselijk perifeer bloed (PBMC’s) kunnen stimuleren . We hebben ook waargenomen dat C8 tumorgroei in CT26 en B16-OVA kon onderdrukken , evenals anti-PD-1 antilichaam-resistent B16-muismodel. De infiltratie van CD8 T-cellen nam significant toe in de micro-omgeving van de tumor, en de IFN-γ-secretie door CD8 ⁺  T-cellen in drainerende lymfeklieren nam ook toe.

Tegelijkertijd maakten we gebruik van modellen voor uitputting van T-cellen en bevestigden we dat C8 anti-tumor effecten uitoefende via het activeren van CD8 ⁺  T-cellen afhankelijke wijze. Het interactiemodel van C8 met hPD-1 werd gesimuleerd en bevestigd door alanine-scanning. Concluderend, C8 vertoont antitumorvermogen door blokkering van PD-1/PD-L1-interactie, en C8 kan een alternatieve kandidaat zijn voor kankerimmunotherapie.

rHu Visfatin
AK9981-1000	Akron Biotech	1mg	Ask for price

Visfatin antibody
10R-1077	Fitzgerald	100 ul	EUR 349
Description: Mouse monoclonal Visfatin antibody

Visfatin antibody
20R-1861	Fitzgerald	100 ug	EUR 673
Description: Rabbit polyclonal Visfatin antibody

Visfatin antibody
20R-1862	Fitzgerald	100 ug	EUR 673
Description: Rabbit polyclonal Visfatin antibody

Visfatin antibody
20R-1863	Fitzgerald	100 ug	EUR 673
Description: Rabbit polyclonal Visfatin antibody

Visfatin antibody
70R-12451	Fitzgerald	100 ug	EUR 403
Description: Rabbit polyclonal Visfatin antibody

Visfatin antibody
70R-12452	Fitzgerald	100 ug	EUR 460
Description: Rabbit polyclonal Visfatin antibody

Visfatin antibody
70R-12453	Fitzgerald	100 ug	EUR 403
Description: Rabbit polyclonal Visfatin antibody

Visfatin protein
30R-2761	Fitzgerald	10 ug	EUR 187
Description: Purified recombinant Human Visfatin protein

Visfatin protein
30R-2762	Fitzgerald	10 ug	EUR 224
Description: Purified recombinant Human Visfatin protein

Visfatin protein
30R-2763	Fitzgerald	10 ug	EUR 281
Description: Purified recombinant Human Visfatin protein

Visfatin protein
30R-AV022	Fitzgerald	25 ug	EUR 273
Description: Purified recombinant Human Visfatin protein

Visfatin antibody
PAab09847	Lifescience Market	100 ug	EUR 386

Visfatin Antibody
5907R-100	Biovision		EUR 316

Visfatin Antibody
5907R-30T	Biovision		EUR 146

Visfatin Antibody
5908-100	Biovision		EUR 354

Visfatin Antibody
5908-30T	Biovision		EUR 146

Visfatin Antibody
5909-100	Biovision		EUR 316

Visfatin Antibody
5909-30T	Biovision		EUR 146

(PPPPNAND)3 peptide (repeat-sequence peptide of the P. berghei circumsporozoite protein, CSP) control/blocking peptide
PPPP321-P	Alpha Diagnostics	100 ug	EUR 164

(NANP)5 peptide (repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) control/blocking peptide
NANP51-P	Alpha Diagnostics	1 mg	EUR 347

Influenza A virus (H5N1) matrix protein 2 hybrid sequence Control/blocking peptide
M2E11-P	Alpha Diagnostics	100 ug	EUR 164

(NVDP)4 peptide (minor repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) control/blocking peptide
NVDP41-P	Alpha Diagnostics	100 ug	EUR 164

(DRAAGQPAG)3 (repeat-sequence peptide of the P. vivax circumsporozoite protein, CSP) control/blocking peptide
DRAA31-P	Alpha Diagnostics	100 ug	EUR 164

Recombinant Visfatin (VF)
4-RPA638Hu01	Cloud-Clone	EUR 458.40 EUR 226.00 EUR 1444.00 EUR 548.00 EUR 996.00 EUR 370.00 EUR 3460.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Visfatin expressed in: E.coli

Recombinant Visfatin (VF)
4-RPA638Mu01	Cloud-Clone	EUR 548.00 EUR 250.00 EUR 1780.00 EUR 660.00 EUR 1220.00 EUR 430.00 EUR 4300.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Visfatin expressed in: E.coli

Recombinant Visfatin (VF)
4-RPA638Ra01	Cloud-Clone	EUR 440.48 EUR 221.00 EUR 1376.80 EUR 525.60 EUR 951.20 EUR 358.00 EUR 3292.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Visfatin expressed in: E.coli

Human Visfatin Antibody
18609-05011	AssayPro	150 ug	EUR 217

Mouse Visfatin Antibody
18715-05011	AssayPro	150 ug	EUR 217

Polyclonal Visfatin Antibody
APR10726G	Leading Biology	0.1mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Visfatin . This antibody is tested and proven to work in the following applications:

Polyclonal Visfatin Antibody
APR10727G	Leading Biology	0.1mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Visfatin . This antibody is tested and proven to work in the following applications:

Polyclonal Visfatin Antibody
APR10728G	Leading Biology	0.1mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Visfatin . This antibody is tested and proven to work in the following applications:

Human Visfatin Antibody
48609-05011	AssayPro	150 ug	EUR 217

Recombinant Human Visfatin
CC143-005	GenDepot	5ug	EUR 293

Recombinant Human Visfatin
CC143-025	GenDepot	25ug	EUR 408

Recombinant Human Visfatin
CC143-101	GenDepot	1mg	EUR 8960

Visfatin (VF) Antibody
20-abx128448	Abbexa	EUR 398.00 EUR 133.00 EUR 1107.00 EUR 537.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug

Visfatin (VF) Antibody
20-abx100120	Abbexa	EUR 425.00 EUR 133.00 EUR 1177.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Visfatin (VF) Antibody
20-abx132001	Abbexa	EUR 328.00 EUR 843.00 EUR 439.00 EUR 154.00 EUR 258.00	100 ug 1 mg 200 ug 20 ug 50 ug

Recombinant Human Visfatin
7-01768	CHI Scientific	5µg	Ask for price

Recombinant Human Visfatin
7-01769	CHI Scientific	25µg	Ask for price

Recombinant Human Visfatin
7-01770	CHI Scientific	1mg	Ask for price

Recombinant Mouse Visfatin
7-01774	CHI Scientific	5µg	Ask for price

Recombinant Mouse Visfatin
7-01775	CHI Scientific	25µg	Ask for price

Recombinant Mouse Visfatin
7-01776	CHI Scientific	1mg	Ask for price

Visfatin Protein (OVA)
20-abx165609	Abbexa	EUR 578.00 EUR 258.00 EUR 1720.00 EUR 690.00 EUR 425.00	100 ug 10 ug 1 mg 200 ug 50 ug

Visfatin (VF) Antibody
20-abx178884	Abbexa	EUR 1135.00 EUR 551.00	1 mg 200 ug

Visfatin (VF) Antibody
20-abx178885	Abbexa	EUR 1135.00 EUR 551.00	1 mg 200 ug

Visfatin (VF) Antibody
20-abx178886	Abbexa	EUR 425.00 EUR 133.00 EUR 1191.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Visfatin (VF) Antibody
20-abx178887	Abbexa	EUR 1233.00 EUR 592.00	1 mg 200 ug

Visfatin (VF) Antibody
20-abx178888	Abbexa	EUR 1288.00 EUR 620.00	1 mg 200 ug

Visfatin (VF) Antibody
20-abx175100	Abbexa	EUR 314.00 EUR 787.00 EUR 411.00 EUR 154.00 EUR 258.00	100 ug 1 mg 200 ug 20 ug 50 ug

Visfatin (VF) Antibody
20-abx175101	Abbexa	EUR 871.00 EUR 453.00	1 mg 200 ug

Visfatin (VF) Antibody
20-abx175102	Abbexa	EUR 913.00 EUR 467.00	1 mg 200 ug

anti- Visfatin antibody
FNab09412	FN Test	100µg	EUR 505.25
Description: Antibody raised against Visfatin

anti- Visfatin antibody
FNab09847	FN Test	100µg	EUR 548.75
Description: Antibody raised against Visfatin

Visfatin ELISA Kit
55R-1414	Fitzgerald	100 assays	EUR 1518
Description: ELISA kit for detection of Visfatin in the research laboratory

Visfatin Recombinant Protein
40-111-0005mg	ProSci	0.005 mg	EUR 259.25
Description: Visfatin is a 55 kDa protein produced and secreted primarily by white adipose tissue. Recently, Visfatin was isolated from visceral fat deposits and shown to possess insulin-mimetic activity. Like insulin, Visfatin exerts hypoglycemic effects by interacting with the insulin receptor. The binding affinity of Visfatin for the insulin receptor is similar to that of insulin, but it does not compete with insulin, suggesting that the two proteins interact with different receptor sites. The circulating levels of Visfatin are much lower than those of insulin and are not affected by feeding, implying that the hypoglycemic effect of Visfatin may not be of physiological importance. The plasma Visfatin levels, like those of Leptin, correlate positively with the percent of body fat and increase during the development of obesity. Another similarity between Visfatin and Leptin is that their amino acid sequence is highly conserved across different mammalian species and shows no homology to any known protein. Receptors for both Leptin (Ob-R) and Visfatin (i.e. the insulin receptor) are expressed by neurons within the arcuate nucleus of the hypothalamus, a brain area that plays a pivotal role in the regulation of energy metabolism. Although the metabolic function of Visfatin is still unknown, it appears that this newly identified adipocytokine might play an important role, similar to that of Leptin, in the regulation of body weight, i.e. as an afferent signal reflecting excess body fat. Recombinant human Visfatin is a 52.5 kDa protein containing 465 amino acid residues (isoform 1).

Visfatin Recombinant Protein
40-111-0025mg	ProSci	0.025 mg	EUR 364.25
Description: Visfatin is a 55 kDa protein produced and secreted primarily by white adipose tissue. Recently, Visfatin was isolated from visceral fat deposits and shown to possess insulin-mimetic activity. Like insulin, Visfatin exerts hypoglycemic effects by interacting with the insulin receptor. The binding affinity of Visfatin for the insulin receptor is similar to that of insulin, but it does not compete with insulin, suggesting that the two proteins interact with different receptor sites. The circulating levels of Visfatin are much lower than those of insulin and are not affected by feeding, implying that the hypoglycemic effect of Visfatin may not be of physiological importance. The plasma Visfatin levels, like those of Leptin, correlate positively with the percent of body fat and increase during the development of obesity. Another similarity between Visfatin and Leptin is that their amino acid sequence is highly conserved across different mammalian species and shows no homology to any known protein. Receptors for both Leptin (Ob-R) and Visfatin (i.e. the insulin receptor) are expressed by neurons within the arcuate nucleus of the hypothalamus, a brain area that plays a pivotal role in the regulation of energy metabolism. Although the metabolic function of Visfatin is still unknown, it appears that this newly identified adipocytokine might play an important role, similar to that of Leptin, in the regulation of body weight, i.e. as an afferent signal reflecting excess body fat. Recombinant human Visfatin is a 52.5 kDa protein containing 465 amino acid residues (isoform 1).

Anti-Visfatin antibody
PAab09412	Lifescience Market	100 ug	EUR 355

Visfatin, human recombinant
4907-10	Biovision		EUR 175

Visfatin, human recombinant
4907-1000	Biovision		EUR 3584

Visfatin, human recombinant
4907-50	Biovision		EUR 468

Visfatin, mouse recombinant
4908-10	Biovision		EUR 175

Visfatin, mouse recombinant
4908-1000	Biovision		EUR 3421

Visfatin, mouse recombinant
4908-50	Biovision		EUR 468

Visfatin, rat recombinant
4909-10	Biovision		EUR 229

Visfatin, rat recombinant
4909-1000	Biovision		EUR 4345

Visfatin, rat recombinant
4909-50	Biovision		EUR 479

C-Peptide Blocking Peptide
3277BP-50	Biovision		EUR 153

Human visfatin ELISA Kit
CSB-E08940h-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human visfatin in samples from serum, plasma, urine, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human visfatin ELISA Kit
1-CSB-E08940h	Cusabio	EUR 900.00 EUR 5476.00 EUR 2900.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human visfatin in samples from serum, plasma, urine, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat visfatin ELISA Kit
CSB-E08941r-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativesandwich ELISA kit for measuring Rat visfatin in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat visfatin ELISA Kit
1-CSB-E08941r	Cusabio	EUR 967.00 EUR 5925.00 EUR 3134.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat visfatin in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse visfatin ELISA Kit
CSB-E08942m-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativesandwich ELISA kit for measuring Mouse visfatin in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse visfatin ELISA Kit
1-CSB-E08942m	Cusabio	EUR 946.00 EUR 5782.00 EUR 3060.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse visfatin in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

OVA conjugated Visfatin (VF)
4-CPA638Hu21	Cloud-Clone	EUR 413.60 EUR 214.00 EUR 1276.00 EUR 492.00 EUR 884.00 EUR 340.00 EUR 3040.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Visfatin expressed in: Protein conjugation

Visfatin Human Recombinant Protein
PROTP43490	BosterBio	Regular: 25ug	EUR 317
Description: Visfatin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 466 amino acids. The total molecular mass is 52.6kDa (calculated). The Visfatin is purified by Flag-affinity chromatography.

Recombinant Human Visfatin Protein
PROTP43490-2	BosterBio	25ug	EUR 317
Description: Visfatin is a 55 kDa protein produced and secreted primarily by white adipose tissue. Recently, Visfatin was isolated from visceral fat deposits and shown to possess insulin-mimetic activity. Like insulin, Visfatin exerts hypoglycemic effects by interacting with the insulin receptor. The binding affinity of Visfatin for the insulin receptor is similar to that of insulin, but it does not compete with insulin, suggesting that the two proteins interact with different receptor sites. The circulating levels of Visfatin are much lower than those of insulin and are not affected by feeding, implying that the hypoglycemic effect of Visfatin may not be of physiological importance. The plasma Visfatin levels, like those of Leptin, correlate positively with the percent of body fat and increase during the development of obesity. Another similarity between Visfatin and Leptin is that their amino acid sequence is highly conserved across different mammalian species and shows no homology to any known protein. Receptors for both Leptin (Ob-R) and Visfatin (i.e. the insulin receptor) are expressed by neurons within the arcuate nucleus of the hypothalamus, a brain area that plays a pivotal role in the regulation of energy metabolism. Although the metabolic function of Visfatin is still unknown, it appears that this newly identified adipocytokine might play an important role, similar to that of Leptin, in the regulation of body weight, i.e. as an afferent signal reflecting excess body fat. Recombinant human Visfatin is a 52.6 kDa protein containing 466 amino acid residues (isoform 1).

Visfatin Mouse Recombinant Protein
PROTQ99KQ4	BosterBio	Regular: 25ug	EUR 317
Description: Visfatin Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (aa 1-491) containing a 20 aa His tag and having 511 amino acids. The total molecular mass is 57kDa. The Visfatin is purified by proprietary chromatographic techniques.

Rat visfatin ELISA Kit
GA-E0479RT-48T	GenAsia Biotech	48T	EUR 317

Rat visfatin ELISA Kit
GA-E0479RT-96T	GenAsia Biotech	96T	EUR 496

Mouse visfatin ELISA Kit
GA-E0610MS-48T	GenAsia Biotech	48T	EUR 336

Mouse visfatin ELISA Kit
GA-E0610MS-96T	GenAsia Biotech	96T	EUR 534

Anti-Visfatin/NAMPT Antibody
PB10009	BosterBio	100ug/vial	EUR 334

Rabbit Visfatin ELISA Kit
ERTV0046	Abclonal	96Tests	EUR 521

Rat Visfatin ELISA Kit
ERV0046	Abclonal	96Tests	EUR 521

Mouse Visfatin ELISA Kit
EMV0046	Abclonal	96Tests	EUR 521

Porcine Visfatin ELISA Kit
EPV0046	Abclonal	96Tests	EUR 521

Goat Visfatin ELISA Kit
EGTV0046	Abclonal	96Tests	EUR 521

Human Visfatin ELISA Kit
EHV0046	Abclonal	96Tests	EUR 521

Bovine Visfatin ELISA Kit
EBV0046	Abclonal	96Tests	EUR 521

Canine Visfatin ELISA Kit
ECV0046	Abclonal	96Tests	EUR 521

Anserini Visfatin ELISA Kit
EAV0046	Abclonal	96Tests	EUR 521

Visfatin (PBEF), His Tagged
PR27140	Neuromics	5 ug	EUR 191

Visfatin / PBEF (NAMPT) Antibody
abx122493-100ug	Abbexa	100 ug	EUR 391

Visfatin / PBEF (NAMPT) Antibody
20-abx114108	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul

Human visfatin ELISA Kit
201-12-0026	SunredBio	96 tests	EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Visfatin / PBEF (NAMPT) Antibody
20-abx320240	Abbexa	EUR 300.00 EUR 244.00	100 ul 50 ul

Visfatin / PBEF (NAMPT) Antibody
20-abx322338	Abbexa	EUR 300.00 EUR 244.00	100 ul 50 ul

Mouse Visfatin (VF) Protein
20-abx652123	Abbexa	EUR 759.00 EUR 300.00 EUR 2388.00 EUR 913.00 EUR 537.00	100 ug 10 ug 1 mg 200 ug 50 ug

Cow Visfatin (VF) Protein
20-abx655491	Abbexa	EUR 648.00 EUR 272.00 EUR 1998.00 EUR 773.00 EUR 467.00	100 ug 10 ug 1 mg 200 ug 50 ug

Human Visfatin (NAMPT) Protein
abx670218-25ug	Abbexa	25 ug	EUR 551

Rat Visfatin (VF) Protein
20-abx069671	Abbexa	EUR 620.00 EUR 272.00 EUR 1859.00 EUR 732.00 EUR 453.00	100 ug 10 ug 1 mg 200 ug 50 ug

Visfatin / PBEF (NAMPT) Antibody
abx034196-400ul	Abbexa	400 ul	EUR 523

Visfatin / PBEF (NAMPT) Antibody
abx034196-80l	Abbexa	80 µl	EUR 286

Visfatin / PBEF (NAMPT) Antibody
abx037068-100ug	Abbexa	100 ug	EUR 391

Visfatin / PBEF (NAMPT) Antibody
20-abx137233	Abbexa	EUR 704.00 EUR 328.00 EUR 230.00	100 ug 20 ug 5 ug

Visfatin / PBEF (NAMPT) Antibody
20-abx000641	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Human Visfatin (VF) Protein
20-abx166522	Abbexa	EUR 648.00 EUR 272.00 EUR 1943.00 EUR 759.00 EUR 467.00	100 ug 10 ug 1 mg 200 ug 50 ug

Visfatin / PBEF (NAMPT) Antibody
abx414754-01mg	Abbexa	0.1 mg	EUR 537

Visfatin / PBEF (NAMPT) Antibody
abx239412-100ug	Abbexa	100 ug	EUR 481

Visfatin / PBEF (NAMPT) Antibody
20-abx242153	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

Visfatin / PBEF (NAMPT) Antibody
20-abx213969	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

Visfatin / PBEF (NAMPT) Antibody
20-abx302448	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Visfatin (VF) Antibody (FITC)
20-abx274125	Abbexa	EUR 467.00 EUR 244.00 EUR 1386.00 EUR 648.00 EUR 356.00	100 ug 10 ug 1 mg 200 ug 50 ug

Visfatin (VF) Antibody (Biotin)
20-abx273196	Abbexa	EUR 453.00 EUR 244.00 EUR 1288.00 EUR 620.00 EUR 342.00	100 ug 10 ug 1 mg 200 ug 50 ug

Visfatin / PBEF (NAMPT) Antibody
20-abx225304	Abbexa	EUR 370.00 EUR 606.00 EUR 314.00	100 ul 200 ul 50 ul

Visfatin, human recombinant protein
P1116-.01	ApexBio	10 µg	EUR 196
Description: Nicotinamide phosphoribosyltransferase (also known as PBEF1 or visfatin) is an enzyme that is encoded by the PBEF1 gene in humans. This protein has also been reported as a cytokine (PBEF) which promotes B cell maturation and inhibits neutrophil apoptosis.

Visfatin, human recombinant protein
P1116-.05	ApexBio	50 µg	EUR 529
Description: Nicotinamide phosphoribosyltransferase (also known as PBEF1 or visfatin) is an enzyme that is encoded by the PBEF1 gene in humans. This protein has also been reported as a cytokine (PBEF) which promotes B cell maturation and inhibits neutrophil apoptosis.

Visfatin, human recombinant protein
P1116-1	ApexBio	1 mg	EUR 4156
Description: Nicotinamide phosphoribosyltransferase (also known as PBEF1 or visfatin) is an enzyme that is encoded by the PBEF1 gene in humans. This protein has also been reported as a cytokine (PBEF) which promotes B cell maturation and inhibits neutrophil apoptosis.

Visfatin, mouse recombinant protein
P1117-.01	ApexBio	10 µg	EUR 203
Description: Visfatin also known as Pre-B cell colony enhancing factor, Nicotinamide phosphoribosyltransferase, Pre-B-cell colony-enhancing factor 1 homolog, NAmPRTase, Nampt, PBEF. Visfatin is a novel adipocytokine produced by visceral fat.

Visfatin, mouse recombinant protein
P1117-.05	ApexBio	50 µg	EUR 529
Description: Visfatin also known as Pre-B cell colony enhancing factor, Nicotinamide phosphoribosyltransferase, Pre-B-cell colony-enhancing factor 1 homolog, NAmPRTase, Nampt, PBEF. Visfatin is a novel adipocytokine produced by visceral fat.

Visfatin, mouse recombinant protein
P1117-1	ApexBio	1 mg	EUR 4156
Description: Visfatin also known as Pre-B cell colony enhancing factor, Nicotinamide phosphoribosyltransferase, Pre-B-cell colony-enhancing factor 1 homolog, NAmPRTase, Nampt, PBEF. Visfatin is a novel adipocytokine produced by visceral fat.

Rat visfatin ELISA Kit
QY-E11173	Qayee Biotechnology	96T	EUR 361

Mouse visfatin ELISA Kit
QY-E21597	Qayee Biotechnology	96T	EUR 361

Horse Visfatin ELISA Kit
EHS0010	FN Test	96T	EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Horse;Sensitivity: 0.094 ng/ml

Bovine Visfatin ELISA Kit
EB0077	FN Test	96T	EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Cattle;Sensitivity: 0.938 ng/ml

Mouse visfatin ELISA Kit
CN-02569M1	ChemNorm	96T	EUR 489

Mouse visfatin ELISA Kit
CN-02569M2	ChemNorm	48T	EUR 338

Human visfatin ELISA Kit
CN-04141H1	ChemNorm	96T	EUR 461

Human visfatin ELISA Kit
CN-04141H2	ChemNorm	48T	EUR 310

Visfatin ELISA Kit| Bovine
EF011029	Lifescience Market	96 Tests	EUR 689

Visfatin ELISA Kit| Equine
EF012664	Lifescience Market	96 Tests	EUR 689

Visfatin protein (His tag)
80R-1028	Fitzgerald	100 ug	EUR 397
Description: Purified recombinant Mouse Visfatin protein

Visfatin protein (His tag)
80R-1045	Fitzgerald	100 ug	EUR 305
Description: Purified recombinant Human Visfatin protein

Visfatin Intracellular ELISA Kit
55R-1416	Fitzgerald	100 assays	EUR 1495
Description: ELISA kit for detection of Intracellular Visfatin in the research laboratory

Equine Visfatin ELISA KIT
ABFN00016	Lifescience Market	96 Tests	EUR 886

Dog Visfatin ELISA kit
E08V0003-192T	BlueGene	192 tests	EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Visfatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Visfatin ELISA kit
E08V0003-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Visfatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Visfatin ELISA kit
E08V0003-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Visfatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Visfatin ELISA kit
E09V0003-192T	BlueGene	192 tests	EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey Visfatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Visfatin ELISA kit
E09V0003-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey Visfatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.