Introduction

Cellufine™ ET clean is Cellufine™ immobilized with poly(ε-lysine) (spherical cellulose beads). The beads bind and remove endotoxin from the sample solution. Poly(ε-lysine) is a microbial poly(amino acid) consisting of 25-35 lysine residues produced by Streptomyces albulus. Poly(ε-lysine) as ligand and cellulose beads as matrix are products of JNC Corporation.

Cellufine™ ET Clean Endotoxin Elimination Beads were co-developed by Kumamoto University and Chisso. Poly(ε-lysine) was immobilized on chloromethyl oxirane-activated cellulose beads. The beads are stable affinity beads that are resistant to cleaning solutions, including 0.2 M sodium hydroxide and 2 M sodium. Cellufine™ ET clean can remove endotoxin from a cell product solution at physiological pH, ionic strength of μ = 0.02-1.0 and 0° -25C°.

Application dates

1. BSA/ETclean L

Column chromatography

• Column size: 1 X 1.1 cm (ID) (1.1 mL)
• Flow rate: 0.17ml/min (10cm/h)
• Buffer: 50 mM PB, pH 7 + 0.15 mol NaCl aq.

Test

• abs protein. at 280nm
• ET LAL rate assay
• Injection sample (150ml)
• BSA 1 mg/ml ET 100 EU/ml

2. Lysozyme / ET clean L

Column chromatography

• Column size: 10 x 0.9 cm (ID) (9.6 mL)
• Flow rate: 0.5ml/min (47cm/h)
• Buffer: 1 mM Tris-HCl, pH 7.3
• Degraded: 0 → 1.0 mol/l aq. NaCl

Test

• abs protein. at 280nm
• ET LAL rate assay
• Injection sample (1ml): 14mg/ml