themoralbrain

Een nieuw bioengineered fragmentpeptide van Vasostatin-1

Een nieuw bioengineered fragmentpeptide van Vasostatin-1 oefent farmacologische activiteiten van gladde spieren en anti-angiogene effecten uit through het blokkeren van de VEGFR-signaleringsroute

Chromogranine A (CgA) is een hydrofiel glycoproteïne dat wordt afgegeven door postganglionaire sympathische neuronen. CgA bestaat uit een enkele peptideketen die talrijke gepaarde basische residuen bevat, die typische splitsingsplaatsen in prohormonen zijn om bioactieve peptiden te genereren. Het wordt erkend als een diagnostische en prognostische serummarker voor neuro-endocriene tumoren. Vasostatine-1 is een van de meest geconserveerde regio’s van CgA en heeft numerous remmende biologische activiteiten. In deze studie werd een nieuw peptidefragment gesynthetiseerd dat drie typische functionele structuren van Vasostatin-1 bevat. Dit unieke bioengineered Vasostatin-1 Derived Peptide (genaamd V1DP) omvat een sterk geconserveerd domein tussen gewervelde soorten in het N-terminale gebied, bestaande uit een disulfidebrug gevormd door twee cysteïneresiduen op respectievelijk aminozuurposities 17 en 38. Trouwens, V1DP bevat twee significante tripeptide-herkenningssequenties: de aminozuurtriplets, RGD en KGD. Onze gegevens toonden aan dat V1DP een dosisafhankelijke relaxatie van de arteriële gladde spier van de rat kon induceren en ook de contractie-activiteit van de gladde spier van de baarmoeder van de rat kon verhogen. Wat nog belangrijker is, we ontdekten dat V1DP de proliferatie van kankercellen remt, de HUVEC-celmigratie moduleert en een anti-angiogenese-effect vertoont, zowel in vitro  en in  vivo. 

We hebben het feitelijke mechanisme van V1DP verder onderzocht en onze resultaten bevestigden dat V1DP de vasculaire endotheliale groeifactorreceptor (VEGFR) -signalering remt. We koppelden V1DP aan de apo-structuren van VEGFR2 en onderzochten de stabiliteit van het peptide in de eiwitpockets. Onze simulatie- en vrije-energieberekeningen gaven aan dat V1DP kan binden aan het katalytische domein en regulatoire domeinzakken, afhankelijk van of de conformationele toestand van het eiwit JM-in of JM-out is. Al met al suggereerden onze gegevens dat V1DP een rol speelt als regulator van de endotheelcelfunctie en farmacologische homeostase van gladde spieren. V1DP is een in water oplosbaar en biologisch stabiel peptide en zou zich verder kunnen ontwikkelen als een anti-angiogeen geneesmiddel voor de behandeling van kanker.

themoralbrain
themoralbrain

 

 

 

 

 

 

 

 

Phospho-BCL-XL (Ser62) Antibody

AF3415 200ul
EUR 304
Description: Phospho-BCL-XL (Ser62) Antibody detects endogenous levels of BCL-XL only when phosphorylated at Serine 62.

Phospho- BCL- XL (Ser62) Antibody

ABF3415 100 ug
EUR 438

anti-BCL-XL (Phospho-Ser62)

LF-PA20051 100 ul
EUR 354
Description: Rabbit polyclonal to BCL-XL (Phospho-Ser62)

BCL-XL (Phospho-Ser62) Polyclonal Conjugated Antibody

C11066 100ul
EUR 397

Phospho-BCL-XL (Thr47) Blocking Peptide

AF3414-BP 1mg
EUR 195

Bcl xL Blocking Peptide

33R-10466 50 ug
EUR 349
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Bcl xL antibody, catalog no. 20R-1516

Bcl-xL Blocking Peptide

33R-10707 50 ug
EUR 191
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Bcl-xL antibody, catalog no. 70R-11750

Bcl-xL Blocking Peptide

3312BP-50
EUR 153

BCL-XL Blocking Peptide

AF6414-BP 1mg
EUR 195

BCL-XL Blocking Peptide

AF6415-BP 1mg
EUR 195

Polyclonal BCL2L1 / BCL-XL Antibody (Ser62)

APG02242G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BCL2L1 / BCL-XL (Ser62). This antibody is tested and proven to work in the following applications:

BCL-XL (Phospho-Ser62) Colorimetric Cell-Based ELISA Kit

EKC2364 100ul
EUR 572

Phospho-Myc (Ser62) Blocking Peptide

AF3054-BP 1mg
EUR 195

Phospho-BCL-XL (Ser62) Colorimetric Cell-Based ELISA Kit (OKAG01898)

OKAG01898 2 x 96 Wells
EUR 740
Description: Description of target: ;Species reactivity: Human: S62, Mouse: S62, Rat: S62;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: Phospho
Detection Method: Colorimetric 450 nm;Sensitivity:

Phospho-BCL-XL (Thr47) Antibody

AF3414 200ul
EUR 304
Description: Phospho-BCL-XL (Thr47) Antibody detects endogenous levels of BCL-XL only when phosphorylated at Threonine 47.

Phospho- BCL- XL (Thr47) Antibody

ABF3414 100 ug
EUR 438

Phospho-ATF2 (Ser62/44) Blocking Peptide

AF3176-BP 1mg
EUR 195

Bcl-x (phospho Ser62) Polyclonal Antibody

ABP56035-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human Bcl-x around the phosphorylation site of S62
  • Applications tips:
Description: A polyclonal antibody for detection of Bcl-x phospho Ser62) from Human, Mouse, Rat. This Bcl-x phospho Ser62) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Bcl-x around the phosphorylation site of S62

Bcl-x (phospho Ser62) Polyclonal Antibody

ABP56035-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human Bcl-x around the phosphorylation site of S62
  • Applications tips:
Description: A polyclonal antibody for detection of Bcl-x phospho Ser62) from Human, Mouse, Rat. This Bcl-x phospho Ser62) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Bcl-x around the phosphorylation site of S62

Bcl-x (phospho Ser62) Polyclonal Antibody

ABP56035-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human Bcl-x around the phosphorylation site of S62
  • Applications tips:
Description: A polyclonal antibody for detection of Bcl-x phospho Ser62) from Human, Mouse, Rat. This Bcl-x phospho Ser62) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Bcl-x around the phosphorylation site of S62

Bcl-x (phospho Ser62) Polyclonal Antibody

ES7034-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Bcl-x (phospho Ser62) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Bcl-x (phospho Ser62) Polyclonal Antibody

ES7034-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Bcl-x (phospho Ser62) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Bcl-xL Antibody

24247-100ul 100ul
EUR 390

Bcl xL antibody

20R-1516 100 ug
EUR 651
Description: Rabbit polyclonal Bcl xL antibody

BCL XL antibody

20R-2242 50 ug
EUR 281
Description: Rabbit polyclonal BCL XL antibody

Bcl-xL antibody

70R-11750 100 ug
EUR 470
Description: Rabbit polyclonal Bcl-xL antibody

Bcl-xL antibody

70R-21426 50 ul
EUR 435
Description: Rabbit polyclonal Bcl-xL antibody

Bcl-xL Antibody

3312-100
EUR 359

Bcl-xL Antibody

3312-30T
EUR 146

Bcl-XL Antibody

48688-100ul 100ul
EUR 333

Bcl-XL Antibody

48688-50ul 50ul
EUR 239

BCL-XL antibody

70R-34417 100 ug
EUR 327
Description: Rabbit polyclonal BCL-XL antibody

BCL-XL Antibody

AF6414 200ul
EUR 304
Description: BCL-XL Antibody detects endogenous levels of total BCL-XL.

BCL-XL Antibody

AF6415 200ul
EUR 304
Description: BCL-XL Antibody detects endogenous levels of total BCL-XL.

BCL- XL Antibody

ABF6414 100 ug
EUR 438

BCL- XL Antibody

ABF6415 100 ug
EUR 438

Phospho-ENSA (Ser67) /ARPP19 (Ser62) Blocking Peptide

AF4381-BP 1mg
EUR 195

Mouse Bcl-XL Antibody

35630-05111 150 ug
EUR 261

BCL-XL antibody (Thr115)

70R-34416 100 ug
EUR 327
Description: Rabbit polyclonal BCL-XL antibody (Thr115)

BCL-XL (pS62) Antibody

20-abx119182
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

BCL-XL (pT115) Antibody

20-abx119349
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

BCL-XL (pS62) Antibody

abx011693-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

BCL-XL (pT47) Antibody

abx011695-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

Bcl-Xl Polyclonal Antibody

EA258-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Bcl-Xl from Human/ Rat/ Mouse. This antibody is tested and validated for IHC

Bcl-Xl Polyclonal Antibody

EA258-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Bcl-Xl from Human/ Rat/ Mouse. This antibody is tested and validated for IHC

Bcl-XL Conjugated Antibody

C48688 100ul
EUR 397

Polyclonal Bcl-xL Antibody

APG02228G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Bcl-xL . This antibody is tested and proven to work in the following applications:

Polyclonal Bcl-xL Antibody

APG02229G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Bcl-xL . This antibody is tested and proven to work in the following applications:

Bcl-Xl Polyclonal Antibody

ABP57290-003ml 0.03ml
EUR 158
  • Immunogen information: Recombinant Protein
  • Applications tips:
Description: A polyclonal antibody for detection of Bcl-Xl from Human, Mouse, Rat. This Bcl-Xl antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

Bcl-Xl Polyclonal Antibody

ABP57290-01ml 0.1ml
EUR 289
  • Immunogen information: Recombinant Protein
  • Applications tips:
Description: A polyclonal antibody for detection of Bcl-Xl from Human, Mouse, Rat. This Bcl-Xl antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

Bcl-Xl Polyclonal Antibody

ABP57290-02ml 0.2ml
EUR 414
  • Immunogen information: Recombinant Protein
  • Applications tips:
Description: A polyclonal antibody for detection of Bcl-Xl from Human, Mouse, Rat. This Bcl-Xl antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

Bcl-Xl Polyclonal Antibody

ES8287-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Bcl-Xl from Human/Mouse/Rat. This antibody is tested and validated for IHC

Bcl-Xl Polyclonal Antibody

ES8287-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Bcl-Xl from Human/Mouse/Rat. This antibody is tested and validated for IHC

Anti-Bcl-Xl antibody

STJ97501 200 µl
EUR 197
Description: Rabbit polyclonal to Bcl-Xl.

Bcl-xl protein

E20-80114 20ug
EUR 408

Bcl-xl protein

E20-80115 20ug
EUR 408

Phospho-BCL-2 (Ser87) Blocking Peptide

AF3138-BP 1mg
EUR 195

Phospho-BCL-2 (Thr69) Blocking Peptide

AF3139-BP 1mg
EUR 195

Phospho-BCL-2 (Ser70) Blocking Peptide

AF3140-BP 1mg
EUR 195

Phospho-Bcl-2 (Thr56) Blocking Peptide

AF4400-BP 1mg
EUR 195

Phospho-BCL-3 (Ser41) Blocking Peptide

AF7256-BP 1mg
EUR 195

Phospho-BCL-10 (Ser138) Blocking Peptide

AF8215-BP 1mg
EUR 195

Phospho-Bcl-6 (Ser343) Blocking Peptide

AF8244-BP 1mg
EUR 195

Phospho-BCL-2 (Thr74) Blocking Peptide

AF8465-BP 1mg
EUR 195

Phospho-BCL-3 (Ser402) Blocking Peptide

AF8466-BP 1mg
EUR 195

Apoptosis Regulator Bcl-X (BCL-XL) Antibody

20-abx011694
  • EUR 439.00
  • EUR 91.00
  • 100 ug
  • 10 ug
  • Shipped within 5-10 working days.

Apoptosis Regulator Bcl-X (BCL-XL) Antibody

abx011696-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

Apoptosis Regulator Bcl-X (BCL-XL) Antibody

abx412040-01mg 0.1 mg
EUR 509
  • Shipped within 1 week.

BCL-XL (Ab-62) Antibody

21061-100ul 100ul
EUR 252

BCL-XL (Ab-62) Antibody

21061-50ul 50ul
EUR 187

Anti-Bcl-XL/BCL2L1 Antibody

A00181 100ug/vial
EUR 334

Bcl-XL recombinant monoclonal antibody

A5091 100ul X 3
EUR 595
  • Comparisons between Mnoclonal, Polyclonal and Recombinant antibodies and their benefits: Regular monoclonal antibodies have higher purity, better specificity and less lot-to-lot variations than polyclonal antibodies. Recombinant antibodies, however,
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Description: A recombinant monoclonal antibody from rabbit against human Bcl-XL for WB, IHC,ELISA

Bcl-XL Rabbit mAb

A19703-100ul 100 ul
EUR 410

Bcl-XL Rabbit mAb

A19703-200ul 200 ul
EUR 571

Bcl-XL Rabbit mAb

A19703-20ul 20 ul
EUR 221

Bcl-XL Rabbit mAb

A19703-50ul 50 ul
EUR 287

pCDH- puro- Bcl- XL

PVT10290 2 ug
EUR 301

Myc (Phospho-Ser62) Antibody

11311-100ul 100ul
EUR 252

Myc (Phospho-Ser62) Antibody

11311-50ul 50ul
EUR 187

Phospho-MYC (Ser62) Antibody

CSB-PA062361-
EUR 335
  • Form: liquid
  • Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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Description: A polyclonal antibody against Phospho-MYC (Ser62). Recognizes Phospho-MYC (Ser62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

Phospho-MYC (Ser62) Antibody

CSB-PA062361-100ul 100ul
EUR 362
  • Form: liquid
  • Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
  • Show more
Description: A polyclonal antibody against Phospho-MYC (Ser62). Recognizes Phospho-MYC (Ser62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

Phospho-BCL2L1 (Ser62) Antibody

CSB-PA786016-
EUR 335
  • Form: liquid
  • Buffer: Purified Rabbit polyclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol. Affinity purification
Description: A polyclonal antibody against Phospho-BCL2L1 (Ser62). Recognizes Phospho-BCL2L1 (Ser62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;

Phospho-BCL2L1 (Ser62) Antibody

CSB-PA786016-100ul 100ul
EUR 362
  • Form: liquid
  • Buffer: Purified Rabbit polyclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol. Affinity purification
Description: A polyclonal antibody against Phospho-BCL2L1 (Ser62). Recognizes Phospho-BCL2L1 (Ser62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;

STMN1 (Phospho-Ser62) Antibody

20-abx012587
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

Phospho-Myc (Ser62) Antibody

AF3054 200ul
EUR 304
Description: Phospho-Myc (Ser62) Antibody detects endogenous levels of Myc only when phosphorylated at Serine 62.

Phospho- Myc (Ser62) Antibody

ABF3054 100 ug
EUR 438

Bcl-2 Blocking Peptide

3033BP-50
EUR 153

Bcl 6 Blocking Peptide

33R-10485 50 ug
EUR 349
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Bcl 6 antibody, catalog no. 20R-1556

Bcl-3 Blocking Peptide

3407BP-50
EUR 153

Bcl-6 Blocking Peptide

3408BP-50
EUR 153

Bcl-Rambo Blocking Peptide

3671BP-50
EUR 153

Bcl-B Blocking Peptide

3695BP-50
EUR 153

BCL-3 Blocking Peptide

DF10317-BP 1mg
EUR 195

BCL-2 Blocking Peptide

AF0769-BP 1mg
EUR 195

Bcl-2 Blocking Peptide

AF4700-BP 1mg
EUR 195

BCL-2 Blocking Peptide

AF6139-BP 1mg
EUR 195

Bcl-2 Blocking Peptide

BF9103-BP 1mg
EUR 195

BCL-3 Blocking Peptide

AF7756-BP 1mg
EUR 195

Mouse Bcl-XL Antibody (Biotin Conjugate)

35630-05121 150 ug
EUR 369

Anti-Bcl-XL Rabbit Monoclonal Antibody

M00181-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Bcl-XL Antibody. Validated in IP, IF, IHC, WB and tested in Human, Mouse, Rat.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D 0.1ml
EUR 354
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is unconjugated.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-A390 0.1ml
EUR 401
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 390.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-A488 0.1ml
EUR 400
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 488.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-A565 0.1ml
EUR 400
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 565.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-A594 0.1ml
EUR 400
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 594.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-A633 0.1ml
EUR 400
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 633.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-A655 0.1ml
EUR 400
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 655.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-A680 0.1ml
EUR 400
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 680.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-A700 0.1ml
EUR 400
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 700.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-ALP 0.1ml
EUR 394
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Alkaline Phosphatase.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-APC 0.1ml
EUR 399
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to APC .

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-APCCY7 0.1ml
EUR 471
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to APC/Cy7.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-BI 0.1ml
EUR 396
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Biotin.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-DY350 0.1ml
EUR 414
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Dylight 350.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-DY405 0.1ml
EUR 403
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Dylight 405.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-DY488 0.1ml
EUR 393
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Dylight 488.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-DY594 0.1ml
EUR 395
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Dylight 594.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-DY633 0.1ml
EUR 390
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Dylight 633.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-FITC 0.1ml
EUR 392
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to FITC.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-HRP 0.1ml
EUR 388
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to HRP.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-P594 0.1ml
EUR 407
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to PE/ATTO 594.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-PCP 0.1ml
EUR 399
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to PerCP.

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-RPE 0.1ml
EUR 397
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to RPE .

Antibody for Human Bcl-xL (pSer62)

SPC-1352D-STR 0.1ml
EUR 398
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Streptavidin.

BCL-XL Cell ELISA Kit

abx595060-96tests 96 tests
EUR 637
  • Shipped within 1-2 weeks.

anti-BCL-XL (Ab-62)

LF-PA20050 100 ul
EUR 334
Description: Rabbit polyclonal to BCL-XL

BCL-XL (Phospho-Thr47) Colorimetric Cell-Based ELISA Kit

EKC2190 100ul
EUR 572

TH (Phospho-Ser62) Polyclonal Antibody

12282-100ul 100ul
EUR 252

TH (Phospho-Ser62) Polyclonal Antibody

12282-50ul 50ul
EUR 187

Phospho-ATF2 (Ser62/44) Antibody

AF3176 200ul
EUR 304
Description: Phospho-ATF2 (Ser62 or 44) Antibody detects endogenous levels of ATF2 only when phosphorylated at Serine 62 or 44.

Op18 (phospho Ser62) Polyclonal Antibody

ABP55090-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human Op18 around the phosphorylation site of S62
  • Applications tips:
Description: A polyclonal antibody for detection of Op18 phospho Ser62) from Human, Mouse, Rat, Monkey. This Op18 phospho Ser62) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Op18 around the phosphorylation site of S62

Op18 (phospho Ser62) Polyclonal Antibody

ABP55090-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human Op18 around the phosphorylation site of S62
  • Applications tips:
Description: A polyclonal antibody for detection of Op18 phospho Ser62) from Human, Mouse, Rat, Monkey. This Op18 phospho Ser62) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Op18 around the phosphorylation site of S62

Op18 (phospho Ser62) Polyclonal Antibody

ABP55090-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human Op18 around the phosphorylation site of S62
  • Applications tips:
Description: A polyclonal antibody for detection of Op18 phospho Ser62) from Human, Mouse, Rat, Monkey. This Op18 phospho Ser62) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Op18 around the phosphorylation site of S62

Ontwikkeling van een peptide dat DNA-herstel remt door de binding van Artemis en DNA Ligase IV te blokkeren om de radiogevoeligheid van tumoren te verbeteren

Doel:  Artemis en DNA-ligase IV zijn twee cruciale elementen in de NHEJ-route van DNA-herstel, die respectievelijk als nuclease en DNA-ligase fungeren. Verbeterde cellulaire stralingsgevoeligheid door remming van beide eiwitten draagt ​​bij aan een veelbelovende benadering om moleculaire gerichte radiosensitizers te ontwikkelen. De interactie tussen Artemis en DNA Ligase IV is vereist voor de activering van Artemis als nuclease bij 3′-overhangend DNA; daarom willen we een remmend peptide genereren dat gericht is op de interactie tussen Artemis en DNA Ligase IV voor de ontwikkeling van nieuwe radiosensitizers.

Methoden:  We hebben het peptide BAL gesynthetiseerd dat bestaat uit de interactieresiduen van Artemis met DNA-ligase IV. Het radiosensibilisatie-effect van BAL werd geëvalueerd door middel van een kolonievormingsassay. De effecten van BAL op door bestraling geïnduceerd DNA-herstel werden geëvalueerd met Western Blotting en immunofluorescentie. Effecten van BAL op celproliferatie, stopzetting van de celcyclus en celapoptose werden beoordeeld through CCK-Eight en flowcytometrie-assays. De mogelijke synergetische effecten van BAL en bestraling in vivo werden onderzocht in een xenograft muismodel.

Resultaten:  Resultaten tonen aan dat het gegenereerde peptide BAL dat de interactie tussen Artemis en DNA Ligase IV blokkeert, de stralingsgevoeligheid van GBC-SD- en Hela-cellijnen important verhoogt. BAL verlengt DNA-herstel na bestraling; BAL en bestraling vertonen synergetische effecten op celproliferatie, celcyclus en celapoptose; en deze functies zijn allemaal gerelateerd aan DNA-ligase IV. Ten slotte hebben we het endogene radiosensibilisatie-effect van BAL in een xenograft-muismodel bevestigd.

Conclusies:  Samenvattend, het remmende peptide BAL dat zich richt op Artemis en DNA Ligase IV-binding functioneert met succes als een nieuwe radiosensitizer die DNA-herstel vertraagt ​​en synergiseert met bestraling om celproliferatie te remmen, celcyclusstilstand te induceren en celapoptose te bevorderen.

Een oraal beschikbare PD-1/PD-L1-blokkerende peptide OPBP-1-geladen trimethylchitosan-hydrogel voor immunotherapie van kanker

Blokkade van het immuuncontrolepunt PD-1/PD-L1 met monoklonale antilichamen toonde ongekende klinische werkzaamheid bij veel kankers. Maar de oraal beschikbare remmers met een laag molecuulgewicht blijven in de kinderschoenen. In vergelijking met kleine moleculen vertoont peptide een betere selectiviteit en minder bijwerkingen, maar een slechte halfwaardetijd en een grote uitdaging om oraal te worden toegediend. Hier hebben we een proteolyse-resistente D-peptide OPBP-1 (Oral PD-L1 Binding Peptide 1) ontwikkeld die selectief PD-L1 kan binden, PD-1/PD-L1-interactie important kan blokkeren en IFN-γ (interferon γ) secretie kan verbeteren van CD8 + T-cellen in menselijke PBMC’s (perifere mononucleaire bloedcellen). OPBP-1 zou de tumorgroei in muriene colorectale CT26- en melanoom B16-OVA-modellen important kunnen remmen bij een relatief lage dosis van 0,5 mg/kg, met verbetering van de infiltratie en functie van CD8 +  T-cellen. Interessanter is dat orale afgifte van OPBP-1 geladen TMC (N, N, N-trimethylchitosan) hydrogel ( [email protected]) vertoonde een zeer goede orale biologische beschikbaarheid van OPBP-1 (52,8%) en verlengde halfwaardetijd (14,55 uur) bij ratten, en remde ook important de tumorgroei in het CT26-model. Concluderend hebben we een PD-1/PD-L1-blokkerend peptide OPBP-1 ontdekt en geoptimaliseerd, through en vervolgens geladen in een op TMC gebaseerd hydrogel oraal toedieningssysteem, om de orale biologische beschikbaarheid van het peptidegeneesmiddel maximaal te verhogen en de tumorgroei effectief te remmen. . Deze resultaten openden een nieuw perspectief voor de ontwikkeling van orale geneesmiddelen bij immunotherapie van kanker.

Intermoleculaire interactieanalyses op SARS-CoV-2 Spike Protein Receptor Binding Area en Human Angiotensine-Changing Enzyme 2 Receptor-blocking Antibody/Peptide met behulp van fragmentmoleculaire orbitale berekening

Het spike-glycoproteïne (S-proteïne) bemiddelt SARS-CoV-2 binnenkomst through intermoleculaire interactie met humaan angiotensine-converterend enzym 2. Het receptorbindende domein (RBD) van het S-proteïne wordt als cruciaal beschouwd voor deze interactie en fungeert als het doelwit van talrijke neutraliserende antilichamen en antivirale peptiden. Deze studie gebruikte de fragment moleculaire orbitale methode om de interacties tussen de RBD en antilichamen/peptiden te analyseren en cruciale residuen te extraheren die als epitopen kunnen worden gebruikt. De interacties geëvalueerd als interfragment interactie energiewaarden tussen de RBD en 12 antilichamen / peptiden toonden een redelijk goede correlatie met de experimentele activiteit pICso 50  ( 2 = 0,540). Negen residuen (T415, Ok417, Y421, F456, A475, F486, N487, N501 en Y505) werden bevestigd als cruciaal. Analyses van de ontleding van de energie van een paar interacties toonden aan dat waterstofbruggen, elektrostatische interacties en π-orbitale interacties belangrijk zijn. Onze resultaten bieden essentiële informatie voor het begrijpen van SARS-CoV-2-antilichaam / peptide-binding en kunnen een rol spelen in het toekomstige ontwerp van antilichaam / antivirale geneesmiddelen.

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