Een nieuw bioengineered fragmentpeptide van Vasostatin-1 oefent farmacologische activiteiten van gladde spieren en anti-angiogene effecten uit through het blokkeren van de VEGFR-signaleringsroute
Chromogranine A (CgA) is een hydrofiel glycoproteïne dat wordt afgegeven door postganglionaire sympathische neuronen. CgA bestaat uit een enkele peptideketen die talrijke gepaarde basische residuen bevat, die typische splitsingsplaatsen in prohormonen zijn om bioactieve peptiden te genereren. Het wordt erkend als een diagnostische en prognostische serummarker voor neuro-endocriene tumoren. Vasostatine-1 is een van de meest geconserveerde regio’s van CgA en heeft numerous remmende biologische activiteiten. In deze studie werd een nieuw peptidefragment gesynthetiseerd dat drie typische functionele structuren van Vasostatin-1 bevat. Dit unieke bioengineered Vasostatin-1 Derived Peptide (genaamd V1DP) omvat een sterk geconserveerd domein tussen gewervelde soorten in het N-terminale gebied, bestaande uit een disulfidebrug gevormd door twee cysteïneresiduen op respectievelijk aminozuurposities 17 en 38. Trouwens, V1DP bevat twee significante tripeptide-herkenningssequenties: de aminozuurtriplets, RGD en KGD. Onze gegevens toonden aan dat V1DP een dosisafhankelijke relaxatie van de arteriële gladde spier van de rat kon induceren en ook de contractie-activiteit van de gladde spier van de baarmoeder van de rat kon verhogen. Wat nog belangrijker is, we ontdekten dat V1DP de proliferatie van kankercellen remt, de HUVEC-celmigratie moduleert en een anti-angiogenese-effect vertoont, zowel in vitro en in vivo.
We hebben het feitelijke mechanisme van V1DP verder onderzocht en onze resultaten bevestigden dat V1DP de vasculaire endotheliale groeifactorreceptor (VEGFR) -signalering remt. We koppelden V1DP aan de apo-structuren van VEGFR2 en onderzochten de stabiliteit van het peptide in de eiwitpockets. Onze simulatie- en vrije-energieberekeningen gaven aan dat V1DP kan binden aan het katalytische domein en regulatoire domeinzakken, afhankelijk van of de conformationele toestand van het eiwit JM-in of JM-out is. Al met al suggereerden onze gegevens dat V1DP een rol speelt als regulator van de endotheelcelfunctie en farmacologische homeostase van gladde spieren. V1DP is een in water oplosbaar en biologisch stabiel peptide en zou zich verder kunnen ontwikkelen als een anti-angiogeen geneesmiddel voor de behandeling van kanker.
Phospho-ATF2 (Ser62/Ser44) Blocking Peptide |
|||
| MBS9615530-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Phospho-ENSA (Ser67/Ser62) Blocking Peptide |
|||
| MBS9616463-1mg | MyBiosource | 1mg | EUR 380 |
Phospho-ENSA (Ser67/Ser62) Blocking Peptide |
|||
| MBS9616463-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Phospho-ATF2 (Ser62/44) Blocking Peptide |
|||
| AF3176-BP | Affbiotech | 1mg | EUR 234 |
Phospho-BCL-XL (Thr47) Blocking Peptide |
|||
| AF3414-BP | Affbiotech | 1mg | EUR 234 |
Phospho-BCL-XL (Thr47) Blocking Peptide |
|||
| MBS9615766-1mg | MyBiosource | 1mg | EUR 380 |
Phospho-BCL-XL (Thr47) Blocking Peptide |
|||
| MBS9615766-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Phospho-Synapsin I (Ser62/Ser67) Blocking Peptide |
|||
| MBS9616065-1mg | MyBiosource | 1mg | EUR 380 |
Phospho-Synapsin I (Ser62/Ser67) Blocking Peptide |
|||
| MBS9616065-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Phospho-ENSA (Ser67) /ARPP19 (Ser62) Blocking Peptide |
|||
| AF4381-BP | Affbiotech | 1mg | EUR 234 |
BCL-2 (Phospho-Ser70) Antibody Blocking Peptide |
|||
| GWB-B2BCC1 | GenWay Biotech | 0.1 mg | Ask for price |
BCL-2 (Phospho-Thr56) Antibody Blocking Peptide |
|||
| GWB-374C85 | GenWay Biotech | 0.1 mg | Ask for price |
Phospho-Bcl-6 (Ser343) Blocking Peptide |
|||
| AF8244-BP | Affbiotech | 1mg | EUR 234 |
Phospho-BCL-3 (Ser402) Blocking Peptide |
|||
| AF8466-BP | Affbiotech | 1mg | EUR 234 |
Phospho-BCL-3 (Ser402) Blocking Peptide |
|||
| MBS9619421-1mg | MyBiosource | 1mg | EUR 380 |
Phospho-BCL-3 (Ser402) Blocking Peptide |
|||
| MBS9619421-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Phospho-BCL-2 (Ser87) Blocking Peptide |
|||
| AF3138-BP | Affbiotech | 1mg | EUR 234 |
Phospho-BCL-2 (Ser70) Blocking Peptide |
|||
| AF3140-BP | Affbiotech | 1mg | EUR 234 |
Phospho-BCL-3 (Ser41) Blocking Peptide |
|||
| AF7256-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Bcl-2 (Ser87) Blocking Peptide |
|||
| MBS9615493-1mg | MyBiosource | 1mg | EUR 380 |
Phospho-Bcl-2 (Ser87) Blocking Peptide |
|||
| MBS9615493-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Phospho-Bcl-2 (Ser70) Blocking Peptide |
|||
| MBS9615495-1mg | MyBiosource | 1mg | EUR 380 |
Phospho-Bcl-2 (Ser70) Blocking Peptide |
|||
| MBS9615495-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Phospho-BCL-3 (Ser41) Blocking Peptide |
|||
| MBS9618336-1mg | MyBiosource | 1mg | EUR 380 |
Phospho-BCL-3 (Ser41) Blocking Peptide |
|||
| MBS9618336-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Phospho-BCL-10 (Ser138) Blocking Peptide |
|||
| AF8215-BP | Affbiotech | 1mg | EUR 234 |
ATF2 (Phospho-Ser62 or 44) Antibody Blocking Peptide |
|||
| GWB-1944B4 | GenWay Biotech | 0.1 mg | Ask for price |
Phospho-NFKBIA(Ser32)) Antibody Blocking peptide |
|||
| MBS9225235-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Phospho-Cofilin(Ser3) Antibody Blocking peptide |
|||
| MBS9225258-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Progesterone Receptor (Phospho- Ser190) Antibody Blocking Peptide |
|||
| GWB-E31CD5 | GenWay Biotech | 0.1 mg | Ask for price |
AMPK1/AMPK2 (Phospho-Ser485/ Ser491) Antibody Blocking Peptide |
|||
| GWB-C21E16 | GenWay Biotech | 0.1 mg | Ask for price |
S6 Ribosomal Protein (Phospho- Ser235) Antibody Blocking Peptide |
|||
| GWB-D0BD8A | GenWay Biotech | 0.1 mg | Ask for price |
IkappaB-alpha (Phospho-Ser32/Ser36) Antibody Blocking Peptide |
|||
| GWB-108697 | GenWay Biotech | 0.1 mg | Ask for price |
Bcl xL Blocking Peptide |
|||
| 33R-10466 | Fitzgerald | 50 ug | EUR 418.8 |
|
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Bcl xL antibody, catalog no. 20R-1516 |
|||
Bcl-xL Blocking Peptide |
|||
| 3312BP-50 | Biovision | each | EUR 183.6 |
Bcl-xL Blocking Peptide |
|||
| 33R-10707 | Fitzgerald | 50 ug | EUR 145 |
|
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Bcl-xL antibody, catalog no. 70R-11750 |
|||
BCL-XL Blocking Peptide |
|||
| AF6414-BP | Affbiotech | 1mg | EUR 234 |
BCL-XL Blocking Peptide |
|||
| AF6415-BP | Affbiotech | 1mg | EUR 234 |
BCL-XL Blocking Peptide |
|||
| MBS9625400-1mg | MyBiosource | 1mg | EUR 380 |
BCL-XL Blocking Peptide |
|||
| MBS9625400-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
BCL-XL Blocking Peptide |
|||
| MBS9617521-1mg | MyBiosource | 1mg | EUR 380 |
BCL-XL Blocking Peptide |
|||
| MBS9617521-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
BCL-XL Blocking Peptide |
|||
| MBS9617522-1mg | MyBiosource | 1mg | EUR 380 |
BCL-XL Blocking Peptide |
|||
| MBS9617522-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
beta-Catenin (Phospho-Thr41/Ser45) Antibody Blocking Peptide |
|||
| GWB-E56609 | GenWay Biotech | 0.1 mg | Ask for price |
Phospho-53BP1 (Ser1778) Blocking Peptide |
|||
| AF8337-BP | Affbiotech | 1mg | EUR 234 |
Phospho-53BP1 (Ser1618) Blocking Peptide |
|||
| AF4460-BP | Affbiotech | 1mg | EUR 234 |
Phospho-53BP1 (Ser1778) Blocking Peptide |
|||
| DF2979-BP | Affbiotech | 1mg | EUR 234 |
Phospho-53BP1 (Ser1778) Blocking Peptide |
|||
| MBS9623161-1mg | MyBiosource | 1mg | EUR 380 |
Phospho-53BP1 (Ser1778) Blocking Peptide |
|||
| MBS9623161-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Phospho-53BP1 (Ser1618) Blocking Peptide |
|||
| MBS9616541-1mg | MyBiosource | 1mg | EUR 380 |
Phospho-53BP1 (Ser1618) Blocking Peptide |
|||
| MBS9616541-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Phospho-53BP1 (Ser1778) Blocking Peptide |
|||
| MBS9619300-1mg | MyBiosource | 1mg | EUR 380 |
Phospho-53BP1 (Ser1778) Blocking Peptide |
|||
| MBS9619300-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Phospho-GR (Ser211) Blocking Peptide |
|||
| AF2004-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Rb (Ser608) Blocking Peptide |
|||
| AF2395-BP | Affbiotech | 1mg | EUR 234 |
Phospho-GR (Ser226) Blocking Peptide |
|||
| AF3066-BP | Affbiotech | 1mg | EUR 234 |
Phospho-GR (Ser203) Blocking Peptide |
|||
| AF3067-BP | Affbiotech | 1mg | EUR 234 |
Phospho-GR(Ser203) Blocking Peptide |
|||
| AF0042-BP | Affbiotech | 1mg | EUR 234 |
Phospho-53BP1 (Ser29) Blocking Peptide |
|||
| AF8236-BP | Affbiotech | 1mg | EUR 234 |
Phospho-53BP1 (Ser29) Blocking Peptide |
|||
| MBS9619199-1mg | MyBiosource | 1mg | EUR 380 |
Phospho-53BP1 (Ser29) Blocking Peptide |
|||
| MBS9619199-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Phospho-TK (Ser13) Blocking Peptide |
|||
| AF3068-BP | Affbiotech | 1mg | EUR 234 |
Phospho-HSL (Ser660) Blocking Peptide |
|||
| AF8026-BP | Affbiotech | 1mg | EUR 234 |
Phospho-ATM (Ser367) Blocking Peptide |
|||
| AF8099-BP | Affbiotech | 1mg | EUR 234 |
Phospho-ATM (Ser183) Blocking Peptide |
|||
| AF8100-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Met (Ser985) Blocking Peptide |
|||
| AF8121-BP | Affbiotech | 1mg | EUR 234 |
Phospho-KIT (Ser746) Blocking Peptide |
|||
| AF8162-BP | Affbiotech | 1mg | EUR 234 |
Phospho-ZAK (Ser165) Blocking Peptide |
|||
| AF8195-BP | Affbiotech | 1mg | EUR 234 |
Phospho-FAK (Ser732) Blocking Peptide |
|||
| AF8227-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Fos (Ser374) Blocking Peptide |
|||
| AF8270-BP | Affbiotech | 1mg | EUR 234 |
Phospho-p53 (Ser392) Blocking Peptide |
|||
| AF8295-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Tel (Ser257) Blocking Peptide |
|||
| AF8325-BP | Affbiotech | 1mg | EUR 234 |
Phospho-XPA (Ser196) Blocking Peptide |
|||
| AF8335-BP | Affbiotech | 1mg | EUR 234 |
Phospho-BTK (Ser179) Blocking Peptide |
|||
| AF8361-BP | Affbiotech | 1mg | EUR 234 |
Phospho-FAK (Ser722) Blocking Peptide |
|||
| AF8373-BP | Affbiotech | 1mg | EUR 234 |
Phospho-ATM (Ser794) Blocking Peptide |
|||
| AF8411-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Cot (Ser400) Blocking Peptide |
|||
| AF8469-BP | Affbiotech | 1mg | EUR 234 |
Phospho-p73 (Ser388) Blocking Peptide |
|||
| AF8500-BP | Affbiotech | 1mg | EUR 234 |
Phospho-VR1 (Ser503) Blocking Peptide |
|||
| AF8520-BP | Affbiotech | 1mg | EUR 234 |
Phospho-YB1 (Ser102) Blocking Peptide |
|||
| AF8525-BP | Affbiotech | 1mg | EUR 234 |
Phospho-CKB(Ser199) Blocking Peptide |
|||
| AF8602-BP | Affbiotech | 1mg | EUR 234 |
phospho-ATR(Ser428) Blocking Peptide |
|||
| DF7512-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Akt(Ser473) Blocking Peptide |
|||
| AF0908-BP | Affbiotech | 1mg | EUR 234 |
Phospho-ULK(Ser317) Blocking Peptide |
|||
| AF2301-BP | Affbiotech | 1mg | EUR 234 |
Phospho-DR6 (Ser562) Blocking Peptide |
|||
| AF2339-BP | Affbiotech | 1mg | EUR 234 |
Phospho-HSL (Ser563) Blocking Peptide |
|||
| AF2350-BP | Affbiotech | 1mg | EUR 234 |
Phospho-MFF (Ser172) Blocking Peptide |
|||
| AF2365-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Syk (Ser297) Blocking Peptide |
|||
| AF2415-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Tau (Ser199) Blocking Peptide |
|||
| AF2418-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Tau (Ser202) Blocking Peptide |
|||
| AF2419-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Tau (Ser416) Blocking Peptide |
|||
| AF2420-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Fos (Ser362) Blocking Peptide |
|||
| AF3053-BP | Affbiotech | 1mg | EUR 234 |
Phospho-p53 (Ser392) Blocking Peptide |
|||
| AF3074-BP | Affbiotech | 1mg | EUR 234 |
Phospho-p53 (Ser366) Blocking Peptide |
|||
| AF3078-BP | Affbiotech | 1mg | EUR 234 |
Phospho-p53 (Ser315) Blocking Peptide |
|||
| AF3079-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Myb (Ser532) Blocking Peptide |
|||
| AF3136-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Tau (Ser214) Blocking Peptide |
|||
| AF3141-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Tau (Ser235) Blocking Peptide |
|||
| AF3142-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Tau (Ser356) Blocking Peptide |
|||
| AF3143-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Tau (Ser404) Blocking Peptide |
|||
| AF3144-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Tau (Ser422) Blocking Peptide |
|||
| AF3145-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Tau (Ser396) Blocking Peptide |
|||
| AF3148-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Tau (Ser262) Blocking Peptide |
|||
| AF3151-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Akt (Ser124) Blocking Peptide |
|||
| AF3260-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Akt (Ser129) Blocking Peptide |
|||
| AF3261-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Akt (Ser473) Blocking Peptide |
|||
| AF3263-BP | Affbiotech | 1mg | EUR 234 |
Phospho-YAP (Ser127) Blocking Peptide |
|||
| AF3328-BP | Affbiotech | 1mg | EUR 234 |
Phospho-AFX (Ser197) Blocking Peptide |
|||
| AF3370-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Rel (Ser503) Blocking Peptide |
|||
| AF3395-BP | Affbiotech | 1mg | EUR 234 |
Phospho-FAK (Ser843) Blocking Peptide |
|||
| AF3401-BP | Affbiotech | 1mg | EUR 234 |
Phospho-HSL (Ser552) Blocking Peptide |
|||
| AF3403-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Kv2.1 (Ser805) Blocking Peptide |
|||
| AF3440-BP | Affbiotech | 1mg | EUR 234 |
Phospho-BAD (Ser155) Blocking Peptide |
|||
| AF3471-BP | Affbiotech | 1mg | EUR 234 |
Phospho-BAD (Ser136) Blocking Peptide |
|||
| AF3472-BP | Affbiotech | 1mg | EUR 234 |
Phospho-VCP (Ser352) Blocking Peptide |
|||
| AF4447-BP | Affbiotech | 1mg | EUR 234 |
Phospho-SRF (Ser103) Blocking Peptide |
|||
| AF4403-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Akt (Ser124) Blocking Peptide |
|||
| AF4418-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Akt(Ser473) Blocking Peptide |
|||
| AF0016-BP | Affbiotech | 1mg | EUR 234 |
Phospho-SKI(Ser383) Blocking Peptide |
|||
| AF7036-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Bim (Ser118) Blocking Peptide |
|||
| AF7121-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Myc (Ser293) Blocking Peptide |
|||
| AF7174-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Bcr (Ser301) Blocking Peptide |
|||
| AF7215-BP | Affbiotech | 1mg | EUR 234 |
Phospho-CBL (Ser804) Blocking Peptide |
|||
| AF7263-BP | Affbiotech | 1mg | EUR 234 |
Phospho-YB1 (Ser176) Blocking Peptide |
|||
| AF7332-BP | Affbiotech | 1mg | EUR 234 |
Phospho-Bad (Ser112) Blocking Peptide |
|||
| AF7427-BP | Affbiotech | 1mg | EUR 234 |
Phospho-ATR (Ser428) Blocking Peptide |
|||
| DF2988-BP | Affbiotech | 1mg | EUR 234 |
Phospho-ATR (Ser428) Blocking Peptide |
|||
| MBS9623169-1mg | MyBiosource | 1mg | EUR 380 |
Phospho-ATR (Ser428) Blocking Peptide |
|||
| MBS9623169-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Phospho-ATR (Ser428) Blocking Peptide |
|||
| MBS9626833-1mg | MyBiosource | 1mg | EUR 380 |
Phospho-ATR (Ser428) Blocking Peptide |
|||
| MBS9626833-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Phospho-p53 (Ser392) Blocking Peptide |
|||
| MBS9615429-1mg | MyBiosource | 1mg | EUR 380 |
Phospho-p53 (Ser392) Blocking Peptide |
|||
| MBS9615429-5x1mg | MyBiosource | 5x1mg | EUR 1650 |
Ontwikkeling van een peptide dat DNA-herstel remt door de binding van Artemis en DNA Ligase IV te blokkeren om de radiogevoeligheid van tumoren te verbeteren
Doel: Artemis en DNA-ligase IV zijn twee cruciale elementen in de NHEJ-route van DNA-herstel, die respectievelijk als nuclease en DNA-ligase fungeren. Verbeterde cellulaire stralingsgevoeligheid door remming van beide eiwitten draagt bij aan een veelbelovende benadering om moleculaire gerichte radiosensitizers te ontwikkelen. De interactie tussen Artemis en DNA Ligase IV is vereist voor de activering van Artemis als nuclease bij 3′-overhangend DNA; daarom willen we een remmend peptide genereren dat gericht is op de interactie tussen Artemis en DNA Ligase IV voor de ontwikkeling van nieuwe radiosensitizers.
Methoden: We hebben het peptide BAL gesynthetiseerd dat bestaat uit de interactieresiduen van Artemis met DNA-ligase IV. Het radiosensibilisatie-effect van BAL werd geëvalueerd door middel van een kolonievormingsassay. De effecten van BAL op door bestraling geïnduceerd DNA-herstel werden geëvalueerd met Western Blotting en immunofluorescentie. Effecten van BAL op celproliferatie, stopzetting van de celcyclus en celapoptose werden beoordeeld through CCK-Eight en flowcytometrie-assays. De mogelijke synergetische effecten van BAL en bestraling in vivo werden onderzocht in een xenograft muismodel.
Resultaten: Resultaten tonen aan dat het gegenereerde peptide BAL dat de interactie tussen Artemis en DNA Ligase IV blokkeert, de stralingsgevoeligheid van GBC-SD- en Hela-cellijnen important verhoogt. BAL verlengt DNA-herstel na bestraling; BAL en bestraling vertonen synergetische effecten op celproliferatie, celcyclus en celapoptose; en deze functies zijn allemaal gerelateerd aan DNA-ligase IV. Ten slotte hebben we het endogene radiosensibilisatie-effect van BAL in een xenograft-muismodel bevestigd.
Conclusies: Samenvattend, het remmende peptide BAL dat zich richt op Artemis en DNA Ligase IV-binding functioneert met succes als een nieuwe radiosensitizer die DNA-herstel vertraagt en synergiseert met bestraling om celproliferatie te remmen, celcyclusstilstand te induceren en celapoptose te bevorderen.
Een oraal beschikbare PD-1/PD-L1-blokkerende peptide OPBP-1-geladen trimethylchitosan-hydrogel voor immunotherapie van kanker
Blokkade van het immuuncontrolepunt PD-1/PD-L1 met monoklonale antilichamen toonde ongekende klinische werkzaamheid bij veel kankers. Maar de oraal beschikbare remmers met een laag molecuulgewicht blijven in de kinderschoenen. In vergelijking met kleine moleculen vertoont peptide een betere selectiviteit en minder bijwerkingen, maar een slechte halfwaardetijd en een grote uitdaging om oraal te worden toegediend. Hier hebben we een proteolyse-resistente D-peptide OPBP-1 (Oral PD-L1 Binding Peptide 1) ontwikkeld die selectief PD-L1 kan binden, PD-1/PD-L1-interactie important kan blokkeren en IFN-γ (interferon γ) secretie kan verbeteren van CD8 + T-cellen in menselijke PBMC’s (perifere mononucleaire bloedcellen). OPBP-1 zou de tumorgroei in muriene colorectale CT26- en melanoom B16-OVA-modellen important kunnen remmen bij een relatief lage dosis van 0,5 mg/kg, met verbetering van de infiltratie en functie van CD8 + T-cellen. Interessanter is dat orale afgifte van OPBP-1 geladen TMC (N, N, N-trimethylchitosan) hydrogel ( OPBP-1@TMC) vertoonde een zeer goede orale biologische beschikbaarheid van OPBP-1 (52,8%) en verlengde halfwaardetijd (14,55 uur) bij ratten, en remde ook important de tumorgroei in het CT26-model. Concluderend hebben we een PD-1/PD-L1-blokkerend peptide OPBP-1 ontdekt en geoptimaliseerd, through en vervolgens geladen in een op TMC gebaseerd hydrogel oraal toedieningssysteem, om de orale biologische beschikbaarheid van het peptidegeneesmiddel maximaal te verhogen en de tumorgroei effectief te remmen. . Deze resultaten openden een nieuw perspectief voor de ontwikkeling van orale geneesmiddelen bij immunotherapie van kanker.
Intermoleculaire interactieanalyses op SARS-CoV-2 Spike Protein Receptor Binding Area en Human Angiotensine-Changing Enzyme 2 Receptor-blocking Antibody/Peptide met behulp van fragmentmoleculaire orbitale berekening
Het spike-glycoproteïne (S-proteïne) bemiddelt SARS-CoV-2 binnenkomst through intermoleculaire interactie met humaan angiotensine-converterend enzym 2. Het receptorbindende domein (RBD) van het S-proteïne wordt als cruciaal beschouwd voor deze interactie en fungeert als het doelwit van talrijke neutraliserende antilichamen en antivirale peptiden. Deze studie gebruikte de fragment moleculaire orbitale methode om de interacties tussen de RBD en antilichamen/peptiden te analyseren en cruciale residuen te extraheren die als epitopen kunnen worden gebruikt. De interacties geëvalueerd als interfragment interactie energiewaarden tussen de RBD en 12 antilichamen / peptiden toonden een redelijk goede correlatie met de experimentele activiteit pICso 50 ( R 2 = 0,540). Negen residuen (T415, Ok417, Y421, F456, A475, F486, N487, N501 en Y505) werden bevestigd als cruciaal. Analyses van de ontleding van de energie van een paar interacties toonden aan dat waterstofbruggen, elektrostatische interacties en π-orbitale interacties belangrijk zijn. Onze resultaten bieden essentiële informatie voor het begrijpen van SARS-CoV-2-antilichaam / peptide-binding en kunnen een rol spelen in het toekomstige ontwerp van antilichaam / antivirale geneesmiddelen.
19 mm Diameter Tapped Titanium Tip |
|||
| 0-120-0012 | Biologics | 19 mm | EUR 639 |
19 mm Diameter Solid Titanium Tip |
|||
| 0-120-0013 | Biologics | 19 mm | EUR 570 |
25 mm Diameter Tapped Titanium Tip |
|||
| 0-120-0014 | Biologics | 25 mm | EUR 682 |
25 mm Diameter Solid Titanium Tip |
|||
| 0-120-0015 | Biologics | 25 mm | EUR 614 |
Flat Replaceable Tips 13 mm Diameter Flat Titanium Tip |
|||
| 0-120-0016 | Biologics | 13 mm | EUR 86 |
|
Description: use with corresponding Tapped Tips and Tapped Extender Tips |
|||
Flat Replaceable Tips 19 mm Diameter Flat Titanium Tip |
|||
| 0-120-0017 | Biologics | 19 mm | EUR 92 |
|
Description: use with corresponding Tapped Tips and Tapped Extender Tips |
|||
Flat Replaceable Tips 25 mm Diameter Flat Titanium Tip |
|||
| 0-120-0018 | Biologics | 25 mm | EUR 100 |
|
Description: use with corresponding Tapped Tips and Tapped Extender Tips |
|||
Titanium Cup Tip, 250 ml |
|||
| 0-120-0019 | Biologics | 250 ml | EUR 1377 |
|
Description: includes Interface Washers |
|||
Microtube Tray, 8 Position (for 250 ml Cup Tip) |
|||
| 0-120-0021 | Biologics | each | EUR 113 |
|
Description: includes Interface Washers |
|||
Continuous Flow Chamber |
|||
| 0-120-0026 | Biologics | each | EUR 774 |
|
Description: includes Interface Washers |
|||
Interface Washers 1.5” Diameter, 5/Pkg |
|||
| 0-120-003 | Biologics | 1.5'' Diameter | EUR 31 |
13 mm Diameter Solid Titanium Extender Tip |
|||
| 0-120-0032 | Biologics | 13 mm | EUR 301 |
13 mm Diameter Tapped Titanium Extender Tip |
|||
| 0-120-0033 | Biologics | 13 mm | EUR 362 |
19 mm Diameter Solid Titanium Extender Tip |
|||
| 0-120-0034 | Biologics | 19 mm | EUR 315 |
19 mm Diameter Tapped Titanium Extender Tip |
|||
| 0-120-0035 | Biologics | 19 mm | EUR 375 |
25 mm Diameter Solid Titanium Extender Tip |
|||
| 0-120-0036 | Biologics | 25 mm | EUR 328 |
25 mm Diameter Tapped Titanium Extender Tip |
|||
| 0-120-0037 | Biologics | 25 mm | EUR 389 |
KoldPod, 1.5ml Micro Tube |
|||
| 0-120-0038 | Biologics | 1.5 ml | EUR 152 |
|
Description: Thermo conductive tube pods |
|||
KoldPod, 15ml Conical Tube |
|||
| 0-120-0039 | Biologics | 15 ml | EUR 275 |
|
Description: Thermo conductive tube pods |
|||
KoldPod, 50ml Conical Tube |
|||
| 0-120-0040 | Biologics | 50 ml | EUR 290 |
|
Description: Thermo conductive tube pods |
|||
Model 150 V/T Ultrasonic Homogenizer |
|||
| 0-121-0002 | Biologics | 210-240V/50-60Hz | EUR 2920 |
|
Description: Delivers up to 150 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample processing processing. |
|||
Model 300 V/T Ultrasonic Homogenizer |
|||
| 0-122-0002 | Biologics | 210-240V/50-60Hz | EUR 3520 |
|
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample. |
|||
SONABOZ Sound Abating Chamber |
|||
| 0-125-0001 | Biologics | each | EUR 1020 |
|
Description: Reduces cavitational sound emitted during processing. |
|||
Model 3000 Ultrasonic Homogenizer |
|||
| 0-127-0002 | Biologics | 210-240V/50-60Hz | EUR 4120 |
|
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip and includes an intergrated Sound Abating Chmaber to reduce cavitational sound emitted during processing. The Timer and Duty Cycle function increase preciosion in sample. |
|||
Model 3000MP Ultrasonic Homogenizer |
|||
| 0-128-0002 | Biologics | 210-240V/50-60Hz | EUR 4720 |
|
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip with preciosion control from a microprocessor and a graphical user interface displayed on a large (145 mm) LCD display. The integrated Sound Abating Chamber reduces cavitational sound emitted during processing. |
|||
OMNICON® Zone Reader, 210-240V/50-60Hz |
|||
| 0-131-0002 | Biologics | 210-240V/50-60Hz | EUR 35200 |
|
Description: Designed to Perform multi-plate Assays on round 90/100mm Petri Dishes. The integrated LED illumination system provides transmitted light for brightfield and darkfield illumination of transparent media. |
|||
OMNI-Noculator Peni Cylinder Filler, 210-240V/50-60Hz |
|||
| 0-134-0002 | Biologics | 210-240V/50-60Hz | EUR 32200 |
|
Description: A robotic liquid handling system designed to dispense Peni Cylinders and fill Peni Cylinders with the corresponding antibiotic liquid sample. |
|||
Peni Cylinder Dispenser with Manual Hopper |
|||
| 0-144-0002 | Biologics | each | EUR 5406 |
|
Description: Dispenser can be configured to dispense 4 or 6 Peni Cylinders onto a petri dish. |
|||
Peni Cylinder Dispenser with Motorized Hopper, 100-240V/50-60Hz |
|||
| 0-144-0003 | Biologics | 100-240V/50-60Hz | EUR 6254 |
|
Description: The motorized hopper can be configured to dispense 4 or 6 Peni Cylinders onto a petri Dispenser can be disassembled for disinfection. |
|||
Stainless Steel Peni Cylinder with Flat Face |
|||
| 0-144-0005 | Biologics | 6mm I.D. x 8mm O.D. x 10mm Long | EUR 399 |
|
Description: sold in packages of 100 pieces |
|||
Stainless Steel Peni Cylinder with Chamfered Face |
|||
| 0-144-0006 | Biologics | 6mm I.D. x 8mm O.D. x 10mm Long | EUR 412 |
|
Description: sold in packages of 100 pieces |
|||
Custom development of ELISAs for other species or antibody isotypes not listed in the catalog. Custom testing of samples for IgG/IgM/IgA or total (IgG+IgM+IgA) |
|||
| 000-CUS | Alpha Diagnostics | Custom | EUR 602 |
Alpha-Bungarotoxin, CF®405S, 500 ug |
|||
| 00002 | Biotium | 1UG | EUR 527 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-bungarotoxin, CF405s |
|||
| 00002 | Cusabio | 500uG | EUR 594 |
|
Description: Minimum order quantity: 1 unit of 500uG |
|||
Alpha-Bungarotoxin, CF®405S, 500 ug |
|||
| 00002-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin, CF®405S 100ug |
|||
| 00002-100ug | Biotium | 100uG | EUR 132 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®680R, 500 ug |
|||
| 9-00003 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®680R, 500 ug |
|||
| 00003-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin, CF®680R 100ug |
|||
| 9-00003 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®640R, 500 ug |
|||
| 9-00004 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®640R, 500 ug |
|||
| 00004-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin, CF®640R 100ug |
|||
| 9-00004 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®488A, 500 ug |
|||
| 9-00005 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®488A, 500 ug |
|||
| 00005-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin CF®488A 100ug |
|||
| 9-00005 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®568, 500 ug |
|||
| 9-00006 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®568, 500 ug |
|||
| 00006-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin, CF®568 100ug |
|||
| 9-00006 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®594, 500 ug |
|||
| 9-00007 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®594, 500 ug |
|||
| 00007-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin CF®594 100ug |
|||
| 9-00007 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®633, 500 ug |
|||
| 9-00009 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®633, 500 ug |
|||
| 00009-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin, CF®633 100ug |
|||
| 9-00009 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
SARS-CoV-2 Indicator Cell Line for RNA Replication - GFP Reporter only |
|||
| 0001-PP-001 | IBT Bioservices | 1 cell line (can order x amount) | EUR 12000 |
|
Description: SARS-CoV-2 GFP reporter cell line using HEK293T (ACE2/TMPRSS2) cells |
|||
Alpha-Bungarotoxin, 1 mg |
|||
| 00010-1 | Biotium | 1MG | EUR 193 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, 1 mg |
|||
| 00010-1-1 | Biotium | EA | EUR 193 |
Fluorescein-Alpha-Bungarotoxin, 500 ug |
|||
| 00011 | Biotium | 500uG | EUR 376 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Fluorescein-Alpha-Bungarotoxin, 500 ug |
|||
| 00011-1 | Biotium | EA | EUR 376 |
Tetramethylrhodamine-Alpha-Bungarotoxin, 500 ug |
|||
| 00012 | Biotium | 500uG | EUR 394 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Tetramethylrhodamine-Alpha-Bungarotoxin, 500 ug |
|||
| 00012-1 | Biotium | EA | EUR 394 |
Fluorescein-alpha-bungarotoxin, 10x50ug |
|||
| 00013 | Biotium | 10ST | EUR 436 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Fluorescein-alpha-bungarotoxin, 10x50ug |
|||
| 00013-1 | Biotium | EA | EUR 436 |
Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug |
|||
| 00014 | Biotium | 10ST | EUR 494 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug |
|||
| 00014-1 | Biotium | EA | EUR 494 |
Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug |
|||
| 00015 | Biotium | 500uG | EUR 494 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug |
|||
| 00015-1 | Biotium | EA | EUR 494 |
Sulforhodamine 101-Alpha-Bungarotoxin, 50 ug |
|||
| 00016 | Biotium | 10ST | EUR 560 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Sulforhodamine 101-Alpha-Bungarotoxin, 50 ug |
|||
| 00016-1 | Biotium | EA | EUR 560 |
Biotin-XX-A-Bungarotoxin, 500 ug |
|||
| 00017 | Biotium | 500uG | EUR 455 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Biotin-XX-A-Bungarotoxin, 500 ug |
|||
| 00017-1 | Biotium | EA | EUR 455 |
Alpha-Bungarotoxin, CF®555, 500 ug |
|||
| 9-00018 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®555, 500 ug |
|||
| 00018-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin, CF®555 100ug |
|||
| 9-00018 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Acrylamide, Chemzymes Ultra Pure® |
|||
| 00019-100 | Polysciences Europe GmbH | 100g | EUR 101.52 |
|
Description: 79-06-1 |
|||
Acrylamide, Chemzymes Ultra Pure® |
|||
| 00019-500 | Polysciences Europe GmbH | 500g | EUR 270 |
|
Description: 79-06-1 |
|||
Biotin-cAMP, 1 mg |
|||
| 00020 | Biotium | 1MG | EUR 298 |
|
Description: N/A |
|||
Biotin-cAMP, 1 mg |
|||
| 00020-1 | Biotium | 20ST | EUR 298 |
|
Description: N/A |
|||
Biotin-cAMP, 50 ug |
|||
| 00020-1-1 | Biotium | EA | EUR 414 |
Biotin-cGMP, 1 mg |
|||
| 00021 | Biotium | 1MG | EUR 331 |
|
Description: N/A |
|||
Biotin-cGMP, 1 mg |
|||
| 00021-1 | Biotium | 20ST | EUR 331 |
|
Description: N/A |
|||
Biotin-cGMP, 20x50 ug |
|||
| 00021-1-1 | Biotium | EA | EUR 447 |
Cyanine 644-cAMP, 1 mg |
|||
| 00022 | Biotium | 1MG | EUR 496 |
|
Description: N/A |
|||
Cyanine 644-cAMP, 1 mg |
|||
| 00022-1 | Biotium | 20ST | EUR 496 |
|
Description: N/A |
|||
Cyanine 644-cAMP, 20x50 ug |
|||
| 00022-1-1 | Biotium | EA | EUR 647 |
Fluorescein Methotrexate, Triammonium Salt, 1 mg |
|||
| 00023 | Biotium | 1MG | EUR 285 |
|
Description: N/A |
|||
Fluorescein Methotrexate, Triammonium Salt, 1 mg |
|||
| 00023-1 | Biotium | EA | EUR 285 |
Staurosporine |
|||
| 00025 | Biotium | 100uG | EUR 100 |
|
Description: N/A |
|||
Staurosporine |
|||
| 00025-1 | Biotium | EA | EUR 100 |
Alpha-Bungarotoxin, CF®543, 500 ug |
|||
| 00026 | Biotium | 500uG | EUR 527 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®543, 500 ug |
|||
| 00026-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin, CF®543, 100 ug |
|||
| 00026-100ug | Biotium | 1UG | EUR 132 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Rhodamine Phalloidin 300U |
|||
| 00027 | Biotium | 300 | EUR 364 |
|
Description: N/A |
|||
Rhodamine Phalloidin 300U |
|||
| 00027-1 | Biotium | EA | EUR 364 |
Biotin-XX-Phalloidin |
|||
| 00028 | Biotium | 100U | EUR 466 |
|
Description: N/A |
|||
Biotin-XX-Phalloidin |
|||
| 00028-1 | Biotium | EA | EUR 466 |
Fluorescein-Phalloidin |
|||
| 00030 | Biotium | 300U | EUR 364 |
|
Description: N/A |
|||
Fluorescein-Phalloidin |
|||
| 00030-1 | Biotium | EA | EUR 364 |
Rhodamine 110 Phalloidin |
|||
| 00032 | Biotium | 300ST | EUR 364 |
|
Description: N/A |
|||
Rhodamine 110 Phalloidin |
|||
| 00032-1 | Biotium | EA | EUR 364 |
Sulforhodamine 101 (Texas Red®) Phalloidin |
|||
| 00033 | Biotium | 300EU | EUR 364 |
|
Description: N/A |
|||
Sulforhodamine 101 (Texas Red®) Phalloidin |
|||
| 00033-1 | Biotium | EA | EUR 364 |
Phalloidin, CF®405M |
|||
| 00034 | Biotium | 300U | EUR 482 |
|
Description: N/A |
|||
Phalloidin, CF®405M |
|||
| 00034-1 | Biotium | EA | EUR 482 |
Phalloidin, CF®405M |
|||
| 00034-T | Biotium | 50U | EUR 101 |
|
Description: N/A |
|||