Een nieuw bioengineered fragmentpeptide van Vasostatin-1 oefent farmacologische activiteiten van gladde spieren en anti-angiogene effecten uit through het blokkeren van de VEGFR-signaleringsroute

Chromogranine A (CgA) is een hydrofiel glycoproteïne dat wordt afgegeven door postganglionaire sympathische neuronen. CgA bestaat uit een enkele peptideketen die talrijke gepaarde basische residuen bevat, die typische splitsingsplaatsen in prohormonen zijn om bioactieve peptiden te genereren. Het wordt erkend als een diagnostische en prognostische serummarker voor neuro-endocriene tumoren. Vasostatine-1 is een van de meest geconserveerde regio’s van CgA en heeft numerous remmende biologische activiteiten. In deze studie werd een nieuw peptidefragment gesynthetiseerd dat drie typische functionele structuren van Vasostatin-1 bevat. Dit unieke bioengineered Vasostatin-1 Derived Peptide (genaamd V1DP) omvat een sterk geconserveerd domein tussen gewervelde soorten in het N-terminale gebied, bestaande uit een disulfidebrug gevormd door twee cysteïneresiduen op respectievelijk aminozuurposities 17 en 38. Trouwens, V1DP bevat twee significante tripeptide-herkenningssequenties: de aminozuurtriplets, RGD en KGD. Onze gegevens toonden aan dat V1DP een dosisafhankelijke relaxatie van de arteriële gladde spier van de rat kon induceren en ook de contractie-activiteit van de gladde spier van de baarmoeder van de rat kon verhogen. Wat nog belangrijker is, we ontdekten dat V1DP de proliferatie van kankercellen remt, de HUVEC-celmigratie moduleert en een anti-angiogenese-effect vertoont, zowel in vitro  en in  vivo. 

We hebben het feitelijke mechanisme van V1DP verder onderzocht en onze resultaten bevestigden dat V1DP de vasculaire endotheliale groeifactorreceptor (VEGFR) -signalering remt. We koppelden V1DP aan de apo-structuren van VEGFR2 en onderzochten de stabiliteit van het peptide in de eiwitpockets. Onze simulatie- en vrije-energieberekeningen gaven aan dat V1DP kan binden aan het katalytische domein en regulatoire domeinzakken, afhankelijk van of de conformationele toestand van het eiwit JM-in of JM-out is. Al met al suggereerden onze gegevens dat V1DP een rol speelt als regulator van de endotheelcelfunctie en farmacologische homeostase van gladde spieren. V1DP is een in water oplosbaar en biologisch stabiel peptide en zou zich verder kunnen ontwikkelen als een anti-angiogeen geneesmiddel voor de behandeling van kanker.

 

 

 

 

 

 

 

 

Phospho-BCL-XL (Ser62) Antibody
AF3415	Affbiotech	200ul	EUR 304
Description: Phospho-BCL-XL (Ser62) Antibody detects endogenous levels of BCL-XL only when phosphorylated at Serine 62.

Phospho- BCL- XL (Ser62) Antibody
ABF3415	Lifescience Market	100 ug	EUR 438

anti-BCL-XL (Phospho-Ser62)
LF-PA20051	Abfrontier	100 ul	EUR 354
Description: Rabbit polyclonal to BCL-XL (Phospho-Ser62)

BCL-XL (Phospho-Ser62) Polyclonal Conjugated Antibody
C11066	SAB	100ul	EUR 397

Phospho-BCL-XL (Thr47) Blocking Peptide
AF3414-BP	Affbiotech	1mg	EUR 195

Bcl xL Blocking Peptide
33R-10466	Fitzgerald	50 ug	EUR 349
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Bcl xL antibody, catalog no. 20R-1516

Bcl-xL Blocking Peptide
33R-10707	Fitzgerald	50 ug	EUR 191
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Bcl-xL antibody, catalog no. 70R-11750

Bcl-xL Blocking Peptide
3312BP-50	Biovision		EUR 153

BCL-XL Blocking Peptide
AF6414-BP	Affbiotech	1mg	EUR 195

BCL-XL Blocking Peptide
AF6415-BP	Affbiotech	1mg	EUR 195

Polyclonal BCL2L1 / BCL-XL Antibody (Ser62)
APG02242G	Leading Biology	0.05mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BCL2L1 / BCL-XL (Ser62). This antibody is tested and proven to work in the following applications:

BCL-XL (Phospho-Ser62) Colorimetric Cell-Based ELISA Kit
EKC2364	BosterBio	100ul	EUR 572

Phospho-Myc (Ser62) Blocking Peptide
AF3054-BP	Affbiotech	1mg	EUR 195

Phospho-BCL-XL (Ser62) Colorimetric Cell-Based ELISA Kit (OKAG01898)
OKAG01898	Aviva Systems Biology	2 x 96 Wells	EUR 740
Description: Description of target: ;Species reactivity: Human: S62, Mouse: S62, Rat: S62;Application: ELISA;Assay info: Assay Type: Cell-Based Subtype: Phospho Detection Method: Colorimetric 450 nm;Sensitivity:

Phospho-BCL-XL (Thr47) Antibody
AF3414	Affbiotech	200ul	EUR 304
Description: Phospho-BCL-XL (Thr47) Antibody detects endogenous levels of BCL-XL only when phosphorylated at Threonine 47.

Phospho- BCL- XL (Thr47) Antibody
ABF3414	Lifescience Market	100 ug	EUR 438

Phospho-ATF2 (Ser62/44) Blocking Peptide
AF3176-BP	Affbiotech	1mg	EUR 195

Bcl-x (phospho Ser62) Polyclonal Antibody
ABP56035-003ml	Abbkine	0.03ml	EUR 158
Immunogen information: Synthesized peptide derived from human Bcl-x around the phosphorylation site of S62 Applications tips:
Description: A polyclonal antibody for detection of Bcl-x phospho Ser62) from Human, Mouse, Rat. This Bcl-x phospho Ser62) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Bcl-x around the phosphorylation site of S62

Bcl-x (phospho Ser62) Polyclonal Antibody
ABP56035-01ml	Abbkine	0.1ml	EUR 289
Immunogen information: Synthesized peptide derived from human Bcl-x around the phosphorylation site of S62 Applications tips:
Description: A polyclonal antibody for detection of Bcl-x phospho Ser62) from Human, Mouse, Rat. This Bcl-x phospho Ser62) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Bcl-x around the phosphorylation site of S62

Bcl-x (phospho Ser62) Polyclonal Antibody
ABP56035-02ml	Abbkine	0.2ml	EUR 414
Immunogen information: Synthesized peptide derived from human Bcl-x around the phosphorylation site of S62 Applications tips:
Description: A polyclonal antibody for detection of Bcl-x phospho Ser62) from Human, Mouse, Rat. This Bcl-x phospho Ser62) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Bcl-x around the phosphorylation site of S62

Bcl-x (phospho Ser62) Polyclonal Antibody
ES7034-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against Bcl-x (phospho Ser62) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Bcl-x (phospho Ser62) Polyclonal Antibody
ES7034-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against Bcl-x (phospho Ser62) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Bcl-xL Antibody
24247-100ul	SAB	100ul	EUR 390

Bcl xL antibody
20R-1516	Fitzgerald	100 ug	EUR 651
Description: Rabbit polyclonal Bcl xL antibody

BCL XL antibody
20R-2242	Fitzgerald	50 ug	EUR 281
Description: Rabbit polyclonal BCL XL antibody

Bcl-xL antibody
70R-11750	Fitzgerald	100 ug	EUR 470
Description: Rabbit polyclonal Bcl-xL antibody

Bcl-xL antibody
70R-21426	Fitzgerald	50 ul	EUR 435
Description: Rabbit polyclonal Bcl-xL antibody

Bcl-xL Antibody
3312-100	Biovision		EUR 359

Bcl-xL Antibody
3312-30T	Biovision		EUR 146

Bcl-XL Antibody
48688-100ul	SAB	100ul	EUR 333

Bcl-XL Antibody
48688-50ul	SAB	50ul	EUR 239

BCL-XL antibody
70R-34417	Fitzgerald	100 ug	EUR 327
Description: Rabbit polyclonal BCL-XL antibody

BCL-XL Antibody
AF6414	Affbiotech	200ul	EUR 304
Description: BCL-XL Antibody detects endogenous levels of total BCL-XL.

BCL-XL Antibody
AF6415	Affbiotech	200ul	EUR 304
Description: BCL-XL Antibody detects endogenous levels of total BCL-XL.

BCL- XL Antibody
ABF6414	Lifescience Market	100 ug	EUR 438

BCL- XL Antibody
ABF6415	Lifescience Market	100 ug	EUR 438

Phospho-ENSA (Ser67) /ARPP19 (Ser62) Blocking Peptide
AF4381-BP	Affbiotech	1mg	EUR 195

Mouse Bcl-XL Antibody
35630-05111	AssayPro	150 ug	EUR 261

BCL-XL antibody (Thr115)
70R-34416	Fitzgerald	100 ug	EUR 327
Description: Rabbit polyclonal BCL-XL antibody (Thr115)

BCL-XL (pS62) Antibody
20-abx119182	Abbexa	EUR 314.00 EUR 98.00 EUR 398.00 EUR 495.00	100 ug 10 ug 200 ug 300 µg
Shipped within 5-10 working days.

BCL-XL (pT115) Antibody
20-abx119349	Abbexa	EUR 314.00 EUR 98.00 EUR 398.00 EUR 495.00	100 ug 10 ug 200 ug 300 µg
Shipped within 5-10 working days.

BCL-XL (pS62) Antibody
abx011693-100ug	Abbexa	100 ug	EUR 439
Shipped within 5-10 working days.

BCL-XL (pT47) Antibody
abx011695-100ug	Abbexa	100 ug	EUR 439
Shipped within 5-10 working days.

Bcl-Xl Polyclonal Antibody
EA258-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against Bcl-Xl from Human/ Rat/ Mouse. This antibody is tested and validated for IHC

Bcl-Xl Polyclonal Antibody
EA258-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against Bcl-Xl from Human/ Rat/ Mouse. This antibody is tested and validated for IHC

Bcl-XL Conjugated Antibody
C48688	SAB	100ul	EUR 397

Polyclonal Bcl-xL Antibody
APG02228G	Leading Biology	0.1 mg	EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Bcl-xL . This antibody is tested and proven to work in the following applications:

Polyclonal Bcl-xL Antibody
APG02229G	Leading Biology	0.1mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Bcl-xL . This antibody is tested and proven to work in the following applications:

Bcl-Xl Polyclonal Antibody
ABP57290-003ml	Abbkine	0.03ml	EUR 158
Immunogen information: Recombinant Protein Applications tips:
Description: A polyclonal antibody for detection of Bcl-Xl from Human, Mouse, Rat. This Bcl-Xl antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

Bcl-Xl Polyclonal Antibody
ABP57290-01ml	Abbkine	0.1ml	EUR 289
Immunogen information: Recombinant Protein Applications tips:
Description: A polyclonal antibody for detection of Bcl-Xl from Human, Mouse, Rat. This Bcl-Xl antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

Bcl-Xl Polyclonal Antibody
ABP57290-02ml	Abbkine	0.2ml	EUR 414
Immunogen information: Recombinant Protein Applications tips:
Description: A polyclonal antibody for detection of Bcl-Xl from Human, Mouse, Rat. This Bcl-Xl antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

Bcl-Xl Polyclonal Antibody
ES8287-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against Bcl-Xl from Human/Mouse/Rat. This antibody is tested and validated for IHC

Bcl-Xl Polyclonal Antibody
ES8287-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against Bcl-Xl from Human/Mouse/Rat. This antibody is tested and validated for IHC

Anti-Bcl-Xl antibody
STJ97501	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Bcl-Xl.

Bcl-xl protein
E20-80114	EnoGene	20ug	EUR 408

Bcl-xl protein
E20-80115	EnoGene	20ug	EUR 408

Phospho-BCL-2 (Ser87) Blocking Peptide
AF3138-BP	Affbiotech	1mg	EUR 195

Phospho-BCL-2 (Thr69) Blocking Peptide
AF3139-BP	Affbiotech	1mg	EUR 195

Phospho-BCL-2 (Ser70) Blocking Peptide
AF3140-BP	Affbiotech	1mg	EUR 195

Phospho-Bcl-2 (Thr56) Blocking Peptide
AF4400-BP	Affbiotech	1mg	EUR 195

Phospho-BCL-3 (Ser41) Blocking Peptide
AF7256-BP	Affbiotech	1mg	EUR 195

Phospho-BCL-10 (Ser138) Blocking Peptide
AF8215-BP	Affbiotech	1mg	EUR 195

Phospho-Bcl-6 (Ser343) Blocking Peptide
AF8244-BP	Affbiotech	1mg	EUR 195

Phospho-BCL-2 (Thr74) Blocking Peptide
AF8465-BP	Affbiotech	1mg	EUR 195

Phospho-BCL-3 (Ser402) Blocking Peptide
AF8466-BP	Affbiotech	1mg	EUR 195

Apoptosis Regulator Bcl-X (BCL-XL) Antibody
20-abx011694	Abbexa	EUR 439.00 EUR 91.00	100 ug 10 ug
Shipped within 5-10 working days.

Apoptosis Regulator Bcl-X (BCL-XL) Antibody
abx011696-100ug	Abbexa	100 ug	EUR 439
Shipped within 5-10 working days.

Apoptosis Regulator Bcl-X (BCL-XL) Antibody
abx412040-01mg	Abbexa	0.1 mg	EUR 509
Shipped within 1 week.

BCL-XL (Ab-62) Antibody
21061-100ul	SAB	100ul	EUR 252

BCL-XL (Ab-62) Antibody
21061-50ul	SAB	50ul	EUR 187

Anti-Bcl-XL/BCL2L1 Antibody
A00181	BosterBio	100ug/vial	EUR 334

Bcl-XL recombinant monoclonal antibody
A5091	Bimake	100ul X 3	EUR 595
Comparisons between Mnoclonal, Polyclonal and Recombinant antibodies and their benefits: Regular monoclonal antibodies have higher purity, better specificity and less lot-to-lot variations than polyclonal antibodies. Recombinant antibodies, however, Show more
Description: A recombinant monoclonal antibody from rabbit against human Bcl-XL for WB, IHC,ELISA

Bcl-XL Rabbit mAb
A19703-100ul	Abclonal	100 ul	EUR 410

Bcl-XL Rabbit mAb
A19703-200ul	Abclonal	200 ul	EUR 571

Bcl-XL Rabbit mAb
A19703-20ul	Abclonal	20 ul	EUR 221

Bcl-XL Rabbit mAb
A19703-50ul	Abclonal	50 ul	EUR 287

pCDH- puro- Bcl- XL
PVT10290	Lifescience Market	2 ug	EUR 301

Myc (Phospho-Ser62) Antibody
11311-100ul	SAB	100ul	EUR 252

Myc (Phospho-Ser62) Antibody
11311-50ul	SAB	50ul	EUR 187

Phospho-MYC (Ser62) Antibody
CSB-PA062361-	Cusabio		EUR 335
Form: liquid Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Show more
Description: A polyclonal antibody against Phospho-MYC (Ser62). Recognizes Phospho-MYC (Ser62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

Phospho-MYC (Ser62) Antibody
CSB-PA062361-100ul	Cusabio	100ul	EUR 362
Form: liquid Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Show more
Description: A polyclonal antibody against Phospho-MYC (Ser62). Recognizes Phospho-MYC (Ser62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

Phospho-BCL2L1 (Ser62) Antibody
CSB-PA786016-	Cusabio		EUR 335
Form: liquid Buffer: Purified Rabbit polyclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol. Affinity purification
Description: A polyclonal antibody against Phospho-BCL2L1 (Ser62). Recognizes Phospho-BCL2L1 (Ser62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;

Phospho-BCL2L1 (Ser62) Antibody
CSB-PA786016-100ul	Cusabio	100ul	EUR 362
Form: liquid Buffer: Purified Rabbit polyclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol. Affinity purification
Description: A polyclonal antibody against Phospho-BCL2L1 (Ser62). Recognizes Phospho-BCL2L1 (Ser62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;

STMN1 (Phospho-Ser62) Antibody
20-abx012587	Abbexa	EUR 314.00 EUR 98.00 EUR 398.00 EUR 495.00	100 ug 10 ug 200 ug 300 µg
Shipped within 5-10 working days.

Phospho-Myc (Ser62) Antibody
AF3054	Affbiotech	200ul	EUR 304
Description: Phospho-Myc (Ser62) Antibody detects endogenous levels of Myc only when phosphorylated at Serine 62.

Phospho- Myc (Ser62) Antibody
ABF3054	Lifescience Market	100 ug	EUR 438

Bcl-2 Blocking Peptide
3033BP-50	Biovision		EUR 153

Bcl 6 Blocking Peptide
33R-10485	Fitzgerald	50 ug	EUR 349
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Bcl 6 antibody, catalog no. 20R-1556

Bcl-3 Blocking Peptide
3407BP-50	Biovision		EUR 153

Bcl-6 Blocking Peptide
3408BP-50	Biovision		EUR 153

Bcl-Rambo Blocking Peptide
3671BP-50	Biovision		EUR 153

Bcl-B Blocking Peptide
3695BP-50	Biovision		EUR 153

BCL-3 Blocking Peptide
DF10317-BP	Affbiotech	1mg	EUR 195

BCL-2 Blocking Peptide
AF0769-BP	Affbiotech	1mg	EUR 195

Bcl-2 Blocking Peptide
AF4700-BP	Affbiotech	1mg	EUR 195

BCL-2 Blocking Peptide
AF6139-BP	Affbiotech	1mg	EUR 195

Bcl-2 Blocking Peptide
BF9103-BP	Affbiotech	1mg	EUR 195

BCL-3 Blocking Peptide
AF7756-BP	Affbiotech	1mg	EUR 195

Mouse Bcl-XL Antibody (Biotin Conjugate)
35630-05121	AssayPro	150 ug	EUR 369

Anti-Bcl-XL Rabbit Monoclonal Antibody
M00181-1	BosterBio	100ug/vial	EUR 397
Description: Rabbit Monoclonal Bcl-XL Antibody. Validated in IP, IF, IHC, WB and tested in Human, Mouse, Rat.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D	Stressmarq	0.1ml	EUR 354
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is unconjugated.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-A390	Stressmarq	0.1ml	EUR 401
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 390.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-A488	Stressmarq	0.1ml	EUR 400
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 488.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-A565	Stressmarq	0.1ml	EUR 400
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 565.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-A594	Stressmarq	0.1ml	EUR 400
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 594.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-A633	Stressmarq	0.1ml	EUR 400
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 633.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-A655	Stressmarq	0.1ml	EUR 400
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 655.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-A680	Stressmarq	0.1ml	EUR 400
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 680.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-A700	Stressmarq	0.1ml	EUR 400
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to ATTO 700.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-ALP	Stressmarq	0.1ml	EUR 394
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Alkaline Phosphatase.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-APC	Stressmarq	0.1ml	EUR 399
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to APC .

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-APCCY7	Stressmarq	0.1ml	EUR 471
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to APC/Cy7.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-BI	Stressmarq	0.1ml	EUR 396
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Biotin.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-DY350	Stressmarq	0.1ml	EUR 414
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Dylight 350.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-DY405	Stressmarq	0.1ml	EUR 403
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Dylight 405.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-DY488	Stressmarq	0.1ml	EUR 393
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Dylight 488.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-DY594	Stressmarq	0.1ml	EUR 395
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Dylight 594.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-DY633	Stressmarq	0.1ml	EUR 390
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Dylight 633.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-FITC	Stressmarq	0.1ml	EUR 392
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to FITC.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-HRP	Stressmarq	0.1ml	EUR 388
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to HRP.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-P594	Stressmarq	0.1ml	EUR 407
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to PE/ATTO 594.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-PCP	Stressmarq	0.1ml	EUR 399
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to PerCP.

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-RPE	Stressmarq	0.1ml	EUR 397
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to RPE .

Antibody for Human Bcl-xL (pSer62)
SPC-1352D-STR	Stressmarq	0.1ml	EUR 398
Description: A polyclonal antibody for Bcl-xL (pSer62) from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human Bcl-xL around the phosphorylation site of serine 62 (pSer62).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This Bcl-xL (pSer62) antibody is conjugated to Streptavidin.

BCL-XL Cell ELISA Kit
abx595060-96tests	Abbexa	96 tests	EUR 637
Shipped within 1-2 weeks.

anti-BCL-XL (Ab-62)
LF-PA20050	Abfrontier	100 ul	EUR 334
Description: Rabbit polyclonal to BCL-XL

BCL-XL (Phospho-Thr47) Colorimetric Cell-Based ELISA Kit
EKC2190	BosterBio	100ul	EUR 572

TH (Phospho-Ser62) Polyclonal Antibody
12282-100ul	SAB	100ul	EUR 252

TH (Phospho-Ser62) Polyclonal Antibody
12282-50ul	SAB	50ul	EUR 187

Phospho-ATF2 (Ser62/44) Antibody
AF3176	Affbiotech	200ul	EUR 304
Description: Phospho-ATF2 (Ser62 or 44) Antibody detects endogenous levels of ATF2 only when phosphorylated at Serine 62 or 44.

Op18 (phospho Ser62) Polyclonal Antibody
ABP55090-003ml	Abbkine	0.03ml	EUR 158
Immunogen information: Synthesized peptide derived from human Op18 around the phosphorylation site of S62 Applications tips:
Description: A polyclonal antibody for detection of Op18 phospho Ser62) from Human, Mouse, Rat, Monkey. This Op18 phospho Ser62) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Op18 around the phosphorylation site of S62

Op18 (phospho Ser62) Polyclonal Antibody
ABP55090-01ml	Abbkine	0.1ml	EUR 289
Immunogen information: Synthesized peptide derived from human Op18 around the phosphorylation site of S62 Applications tips:
Description: A polyclonal antibody for detection of Op18 phospho Ser62) from Human, Mouse, Rat, Monkey. This Op18 phospho Ser62) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Op18 around the phosphorylation site of S62

Op18 (phospho Ser62) Polyclonal Antibody
ABP55090-02ml	Abbkine	0.2ml	EUR 414
Immunogen information: Synthesized peptide derived from human Op18 around the phosphorylation site of S62 Applications tips:
Description: A polyclonal antibody for detection of Op18 phospho Ser62) from Human, Mouse, Rat, Monkey. This Op18 phospho Ser62) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Op18 around the phosphorylation site of S62

Ontwikkeling van een peptide dat DNA-herstel remt door de binding van Artemis en DNA Ligase IV te blokkeren om de radiogevoeligheid van tumoren te verbeteren

Doel:  Artemis en DNA-ligase IV zijn twee cruciale elementen in de NHEJ-route van DNA-herstel, die respectievelijk als nuclease en DNA-ligase fungeren. Verbeterde cellulaire stralingsgevoeligheid door remming van beide eiwitten draagt ​​bij aan een veelbelovende benadering om moleculaire gerichte radiosensitizers te ontwikkelen. De interactie tussen Artemis en DNA Ligase IV is vereist voor de activering van Artemis als nuclease bij 3′-overhangend DNA; daarom willen we een remmend peptide genereren dat gericht is op de interactie tussen Artemis en DNA Ligase IV voor de ontwikkeling van nieuwe radiosensitizers.

Methoden:  We hebben het peptide BAL gesynthetiseerd dat bestaat uit de interactieresiduen van Artemis met DNA-ligase IV. Het radiosensibilisatie-effect van BAL werd geëvalueerd door middel van een kolonievormingsassay. De effecten van BAL op door bestraling geïnduceerd DNA-herstel werden geëvalueerd met Western Blotting en immunofluorescentie. Effecten van BAL op celproliferatie, stopzetting van de celcyclus en celapoptose werden beoordeeld through CCK-Eight en flowcytometrie-assays. De mogelijke synergetische effecten van BAL en bestraling in vivo werden onderzocht in een xenograft muismodel.

Resultaten:  Resultaten tonen aan dat het gegenereerde peptide BAL dat de interactie tussen Artemis en DNA Ligase IV blokkeert, de stralingsgevoeligheid van GBC-SD- en Hela-cellijnen important verhoogt. BAL verlengt DNA-herstel na bestraling; BAL en bestraling vertonen synergetische effecten op celproliferatie, celcyclus en celapoptose; en deze functies zijn allemaal gerelateerd aan DNA-ligase IV. Ten slotte hebben we het endogene radiosensibilisatie-effect van BAL in een xenograft-muismodel bevestigd.

Conclusies:  Samenvattend, het remmende peptide BAL dat zich richt op Artemis en DNA Ligase IV-binding functioneert met succes als een nieuwe radiosensitizer die DNA-herstel vertraagt ​​en synergiseert met bestraling om celproliferatie te remmen, celcyclusstilstand te induceren en celapoptose te bevorderen.

Een oraal beschikbare PD-1/PD-L1-blokkerende peptide OPBP-1-geladen trimethylchitosan-hydrogel voor immunotherapie van kanker

Blokkade van het immuuncontrolepunt PD-1/PD-L1 met monoklonale antilichamen toonde ongekende klinische werkzaamheid bij veel kankers. Maar de oraal beschikbare remmers met een laag molecuulgewicht blijven in de kinderschoenen. In vergelijking met kleine moleculen vertoont peptide een betere selectiviteit en minder bijwerkingen, maar een slechte halfwaardetijd en een grote uitdaging om oraal te worden toegediend. Hier hebben we een proteolyse-resistente D-peptide OPBP-1 (Oral PD-L1 Binding Peptide 1) ontwikkeld die selectief PD-L1 kan binden, PD-1/PD-L1-interactie important kan blokkeren en IFN-γ (interferon γ) secretie kan verbeteren van CD8 ⁺ T-cellen in menselijke PBMC’s (perifere mononucleaire bloedcellen). OPBP-1 zou de tumorgroei in muriene colorectale CT26- en melanoom B16-OVA-modellen important kunnen remmen bij een relatief lage dosis van 0,5 mg/kg, met verbetering van de infiltratie en functie van CD8 ⁺  T-cellen. Interessanter is dat orale afgifte van OPBP-1 geladen TMC (N, N, N-trimethylchitosan) hydrogel ( [email protected]) vertoonde een zeer goede orale biologische beschikbaarheid van OPBP-1 (52,8%) en verlengde halfwaardetijd (14,55 uur) bij ratten, en remde ook important de tumorgroei in het CT26-model. Concluderend hebben we een PD-1/PD-L1-blokkerend peptide OPBP-1 ontdekt en geoptimaliseerd, through en vervolgens geladen in een op TMC gebaseerd hydrogel oraal toedieningssysteem, om de orale biologische beschikbaarheid van het peptidegeneesmiddel maximaal te verhogen en de tumorgroei effectief te remmen. . Deze resultaten openden een nieuw perspectief voor de ontwikkeling van orale geneesmiddelen bij immunotherapie van kanker.

Intermoleculaire interactieanalyses op SARS-CoV-2 Spike Protein Receptor Binding Area en Human Angiotensine-Changing Enzyme 2 Receptor-blocking Antibody/Peptide met behulp van fragmentmoleculaire orbitale berekening

Het spike-glycoproteïne (S-proteïne) bemiddelt SARS-CoV-2 binnenkomst through intermoleculaire interactie met humaan angiotensine-converterend enzym 2. Het receptorbindende domein (RBD) van het S-proteïne wordt als cruciaal beschouwd voor deze interactie en fungeert als het doelwit van talrijke neutraliserende antilichamen en antivirale peptiden. Deze studie gebruikte de fragment moleculaire orbitale methode om de interacties tussen de RBD en antilichamen/peptiden te analyseren en cruciale residuen te extraheren die als epitopen kunnen worden gebruikt. De interacties geëvalueerd als interfragment interactie energiewaarden tussen de RBD en 12 antilichamen / peptiden toonden een redelijk goede correlatie met de experimentele activiteit pICso ₅₀  ( R ² = 0,540). Negen residuen (T415, Ok417, Y421, F456, A475, F486, N487, N501 en Y505) werden bevestigd als cruciaal. Analyses van de ontleding van de energie van een paar interacties toonden aan dat waterstofbruggen, elektrostatische interacties en π-orbitale interacties belangrijk zijn. Onze resultaten bieden essentiële informatie voor het begrijpen van SARS-CoV-2-antilichaam / peptide-binding en kunnen een rol spelen in het toekomstige ontwerp van antilichaam / antivirale geneesmiddelen.

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